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Research article - Peer-reviewed, 2021

Induction of Plant Resistance in Tobacco (Nicotiana tabacum) against Tomato Spotted Wilt Orthotospovirus through Foliar Application of dsRNA

Konakalla, Naga Charan; Bag, Sudeep; Deraniyagala, Anushi Suwaneththiya; Culbreath, Albert K.; Pappu, Hanu R.

Abstract

Thrips-transmitted tomato spotted wilt orthotospovirus (TSWV) continues to be a constraint to peanut, pepper, tobacco, and tomato production in Georgia and elsewhere. TSWV is being managed by an integrated disease management strategy that includes a combination of cultural practices, vector management, and growing virus-resistant varieties where available. We used a non-transgenic strategy to induce RNA interference (RNAi)-mediated resistance in tobacco (Nicotiana tabacum) plants against TSWV. Double-stranded RNA (dsRNA) molecules for the NSs (silencing suppressor) and N (nucleoprotein) genes were produced by a two-step PCR approach followed by in vitro transcription. When topically applied to tobacco leaves, both molecules elicited a resistance response. Host response to the treatments was measured by determining the time to symptom expression, and the level of resistance by absolute quantification of the virus. We also show the systemic movement of dsRNA_N from the inoculated leaves to younger, non-inoculated leaves. Post-application, viral siRNAs were detected for up to nine days in inoculated leaves and up to six days in non-inoculated leaves. The topical application of dsRNAs to induce RNAi represents an environmentally safe and efficient way to manage TSWV in tobacco crops and could be applicable to other TSWV-susceptible crops.

Keywords

double-stranded RNA; tomato spotted wilt virus; N gene; NSs gene; viral suppressors of RNA silencing; RNA interference; vsiRNA; virus resistance

Published in

Viruses
2021, volume: 13, number: 4, article number: 662
Publisher: MDPI

Authors' information

Konakalla, Nagacharan
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Konakalla, Nagacharan
University of Georgia
Bag, Sudeep
University System of Georgia
Deraniyagala, Anushi Suwaneththiya
University System of Georgia
Culbreath, Albert K.
University System of Georgia
Pappu, Hanu R.
Washington State University

UKÄ Subject classification

Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.3390/v13040662

URI (permanent link to this page)

https://res.slu.se/id/publ/111924