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Research article2021Peer reviewedOpen access

Pickle Recruits Retinoblastoma Related 1 to Control Lateral Root Formation in Arabidopsis

Otvos, Krisztina; Miskolczi, Pal; Marhavy, Peter; Cruz-Ramirez, Alfredo; Benkova, Eva; Robert, Stephanie; Bako, Laszlo


Lateral root (LR) formation is an example of a plant post-embryonic organogenesis event. LRs are issued from non-dividing cells entering consecutive steps of formative divisions, proliferation and elongation. The chromatin remodeling protein PICKLE (PKL) negatively regulates auxin-mediated LR formation through a mechanism that is not yet known. Here we show that PKL interacts with RETINOBLASTOMA-RELATED 1 (RBR1) to repress the LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16) promoter activity. Since LBD16 function is required for the formative division of LR founder cells, repression mediated by the PKL-RBR1 complex negatively regulates formative division and LR formation. Inhibition of LR formation by PKL-RBR1 is counteracted by auxin, indicating that, in addition to auxin-mediated transcriptional responses, the fine-tuned process of LR formation is also controlled at the chromatin level in an auxin-signaling dependent manner.


chromatin remodeling; auxin signaling; de novo organogenesis

Published in

International Journal of Molecular Sciences
2021, Volume: 22, number: 8, article number: 3862
Publisher: MDPI