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Research article - Peer-reviewed, 2021

A Rapid and Sensitive Europium Nanoparticle-Based Lateral Flow Immunoassay Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Three Food-Borne Pathogens

Chen, Kai; Ma, Biao; Li, Jiali; Chen, Erjing; Xu, Ying; Yu, Xiaoping; Sun, Chuanxin; Zhang, Mingzhou

Abstract

Food-borne pathogens have become an important public threat to human health. There are many kinds of pathogenic bacteria in food consumed daily. A rapid and sensitive testing method for multiple food-borne pathogens is essential. Europium nanoparticles (EuNPs) are used as fluorescent probes in lateral flow immunoassays (LFIAs) to improve sensitivity. Here, recombinase polymerase amplification (RPA) combined with fluorescent LFIA was established for the simultaneous and quantitative detection of Listeria monocytogenes, Vibrio parahaemolyticus, and Escherichia coli O157:H7. In this work, the entire experimental process could be completed in 20 min at 37 degrees C. The limits of detection (LODs) of EuNP-based LFIA-RPA were 9.0 colony-forming units (CFU)/mL for Listeria monocytogenes, 7.0 CFU/mL for Vibrio parahaemolyticus, and 4.0 CFU/mL for Escherichia coli O157:H7. No cross-reaction could be observed in 22 bacterial strains. The fluorescent LFIA-RPA assay exhibits high sensitivity and good specificity. Moreover, the average recovery of the three food-borne pathogens spiked in food samples was 90.9-114.2%. The experiments indicate the accuracy and reliability of the multiple fluorescent test strips. Our developed EuNP-based LFIA-RPA assay is a promising analytical tool for the rapid and simultaneous detection of multiple low concentrations of food-borne pathogens.

Keywords

recombinase polymerase amplification; europium nanoparticles; lateral flow immunoassay; multiplex detection; food-borne pathogens

Published in

International journal of environmental research and public health
2021, volume: 18, number: 9, article number: 4574
Publisher: MDPI

Authors' information

Chen, Kai
China Jiliang University
Ma, Biao
China Jiliang University
Li, Jiali
China Jiliang University
Chen, Erjing
China Jiliang University
Xu, Ying
China Jiliang University
Yu, Xiaoping
China Jiliang University
Swedish University of Agricultural Sciences, Department of Plant Biology
Zhang, Mingzhou
China Jiliang University

UKÄ Subject classification

Microbiology in the medical area
Public Health, Global Health, Social Medicine and Epidemiology

Publication Identifiers

DOI: https://doi.org/10.3390/ijerph18094574

URI (permanent link to this page)

https://res.slu.se/id/publ/111980