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Research article - Peer-reviewed, 2021

Acute hepatopancreatic necrosis disease (AHPND) toxin degradation by Bacillus subtilis DSM33018

Nguyen, Ngoc Diem; Pande, Gde Sasmita Julyantoro; Abul Kashem, Md; Baruah, Kartik; Bossier, Peter

Abstract

Early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND), results in significant mortality in penaeid shrimp aquaculture. AHPND is caused by toxins secreted by pathogenic strains of Vibrio parahaemolyticus, that have acquired a unique 63-70 kb AHPND-associated plasmid (pVA1). This plasmid encodes the binary PirA/BVP toxins that consist of two subunits PirAVP and PirBVP. Consequently, the degradation of these toxins might be a valid strategy to control or mitigate AHPND. There is literature-based evidence that the application of Bacillus improves shrimp survival upon challenge with pathogenic V. parahaemolyticus. In this study, Bacillus subtilis DSM33018 strain was shown to degrade AHPND toxins in vitro, as detected by Western blots. Further, an in vivo challenge test, using gnotobiotically cultured brine shrimp Artemia franciscana exposed to PirA/BVP toxins, the DSM33018 Bacillus strain could completely alleviate toxicity. This finding indicates that mitigation of AHPND might include Bacillus-based degradation of PirVP toxins.

Keywords

AHPND; Bacillus subtilis; Shrimp; Degradation; PirA; BVP toxin

Published in

Aquaculture
2021, Volume: 540, article number: 736634
Publisher: ELSEVIER

    UKÄ Subject classification

    Fish and Aquacultural Science

    Publication identifier

    DOI: https://doi.org/10.1016/j.aquaculture.2021.736634

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/111985