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Research article - Peer-reviewed, 2022

Fluorescence activated cell sorting-A selective tool for plant cell isolation and analysis

Antoniadi, Ioanna; Skalicky, Vladimir; Sun, Guiling; Ma, Wen; Galbraith, David W.; Novak, Ondrej; Ljung, Karin

Abstract

Instrumentation for flow cytometry and sorting is designed around the assumption that samples are single-cell suspensions. However, with few exceptions, higher plants comprise complex multicellular tissues and organs, in which the individual cells are held together by shared cell walls. Single-cell suspensions can be obtained through digestion of the cells walls and release of the so-called protoplasts (plants without their cell wall). Here we describe best practices for protoplast preparation, and for analysis through flow cytometry and cell sorting. Finally, the numerous downstream applications involving sorted protoplasts are discussed.

Keywords

autofluorescence; best practices; plant flow cytometry and sorting; protoplasts; viability and integrity

Published in

Cytometry Part A
2022, volume: 101, number: 9, pages: 725-736
Publisher: WILEY

Authors' information

Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Skalicky, Vladimir
Palacky University Olomouc
Sun, Guiling
Henan University
Ma, Wen
Henan University
Galbraith, David W.
University of Arizona
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Palacký University Olomouc
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology

UKÄ Subject classification

Cell Biology

Publication Identifiers

DOI: https://doi.org/10.1002/cyto.a.24461

URI (permanent link to this page)

https://res.slu.se/id/publ/112097