Fluorescence activated cell sorting-A selective tool for plant cell isolation and analysis

Abstract

Instrumentation for flow cytometry and sorting is designed around the assumption that samples are single-cell suspensions. However, with few exceptions, higher plants comprise complex multicellular tissues and organs, in which the individual cells are held together by shared cell walls. Single-cell suspensions can be obtained through digestion of the cells walls and release of the so-called protoplasts (plants without their cell wall). Here we describe best practices for protoplast preparation, and for analysis through flow cytometry and cell sorting. Finally, the numerous downstream applications involving sorted protoplasts are discussed.

Keywords

autofluorescence; best practices; plant flow cytometry and sorting; protoplasts; viability and integrity

Published in

Cytometry Part A
2022, Volume: 101, number: 9, pages: 725-736
Publisher: WILEY

SLU Authors

• Antoniadi, Ioanna

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Novak, Ondrej

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    
  • Palacký University Olomouc

• Ljung, Karin

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Cell Biology


Publication identifier

DOI: https://doi.org/10.1002/cyto.a.24461

Permanent link to this page (URI)

https://res.slu.se/id/publ/112097