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Research article - Peer-reviewed, 2021

Comparative analysis of swine leukocyte antigen gene diversity in European farmed pigs

Hammer, S. E.; Duckova, T.; Groiss, S.; Stadler, M.; Jensen-Waern, M.; Golde, W. T.; Gimsa, U.; Saalmueller, A.

Abstract

In Europe, swine represent economically important farm animals and furthermore have become a preferred preclinical large animal model for biomedical studies, transplantation and regenerative medicine research. The need for typing of the swine leukocyte antigen (SLA) is increasing with the expanded use of pigs as models for human diseases and organ-transplantation experiments and their use in infection studies and for design of veterinary vaccines. In this study, we characterised the SLA class I (SLA-1, SLA-2, SLA-3) and class II (DRB1, DQB1, DQA) genes of 549 farmed pigs representing nine commercial pig lines by low-resolution (Lr) SLA haplotyping. In total, 50 class I and 37 class II haplotypes were identified in the studied cohort. The most common SLA class I haplotypes Lr-04.0 (SLA-1*04XX-SLA-3*04XX(04:04)-SLA-2*04XX) and Lr-32.0 (SLA-1*07XX-SLA-3*04XX(04:04)-SLA-2*02XX) occurred at frequencies of 11.02 and 8.20% respectively. For SLA class II, the most prevalent haplotypes Lr-0.15b (DRB1*04XX(04:05/04:06)-DQB1*02XX(02:02)-DQA*02XX) and Lr-0.12 (DRB1*06XX-DQB1*07XX-DQA*01XX) occurred at frequencies of 14.37 and 12.46% respectively. Meanwhile, our laboratory has contributed to several vaccine correlation studies (e.g. Porcine Reproductive and Respiratory Syndrome Virus, Classical Swine Fever Virus, Foot-and-Mouth Disease Virus and Swine Influenza A Virus) elucidating the immunodominance in the T-cell response with antigen specificity dependent on certain SLA-I and SLA-II haplotypes. Moreover, these SLA-immune response correlations could facilitate tailored vaccine development, as SLA-I Lr-04.0 and Lr-32.0 as well as SLA-II Lr-0.15b and Lr-0.12 are highly abundant haplotypes in European farmed pigs.

Keywords

polymorphism; sequence‐specific primers PCR; Sus scrofa; swine leukocyte antigen

Published in

Animal Genetics
2021, volume: 52, number: 4, pages: 523-531
Publisher: WILEY

Authors' information

Hammer, S. E.
University of Veterinary Medicine Vienna
Duckova, T.
University of Veterinary Medicine Vienna
Groiss, S.
University of Veterinary Medicine Vienna
Stadler, M.
University of Veterinary Medicine Vienna
Jensen-Waern, Marianne (Jensen Waern, Marianne)
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Golde, W. T.
Moredun Research Institute
Gimsa, U.
Leibniz Institut fur Nutztierbiologie (FBN)
Saalmueller, A.
University of Veterinary Medicine Vienna

UKÄ Subject classification

Animal and Dairy Science

Publication Identifiers

DOI: https://doi.org/10.1111/age.13090

URI (permanent link to this page)

https://res.slu.se/id/publ/112098