Sundaramoorthy, Jagadeesh
- Department of Plant Breeding, Swedish University of Agricultural Sciences
- Kyungpook National University
Abstract
The enzyme flavonoid 3',5'-hydroxylase (F3'5'H) plays an important role in producing anthocyanin pigments in soybean. Loss of function of the W1 locus encoding F3'5'H always produces white flowers. However, few color variations have been reported in wild soybean. In the present study, we isolated a new color variant of wild soybean accession (IT261811) with pinkish-white flowers. We found that the flower's pinkish-white color is caused by w1-s3, a single recessive allele of W1. The SNP detected in the mutant caused amino acid substitution (A(304)S) in a highly conserved SRS4 domain of F3'5'H proteins. On the basis of the results of the protein variation effect analyzer (PROVEAN) tool, we suggest that this mutation may lead to hypofunctional F3'5'H activity rather than non-functional activity, which thereby results in its pinkish-white color.
Keywords
flavonoid 3 ',5 '-hydroxylase; Glycine soja; pinkish-white flower; soybean; W1 locus
Published in
Agronomy
2021, volume: 11, number: 5, article number: 1001
Publisher: MDPI
SLU Authors
UKÄ Subject classification
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.3390/agronomy11051001
Permanent link to this page (URI)
https://res.slu.se/id/publ/112135