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Research article - Peer-reviewed, 2021

A Novel Pinkish-White Flower Color Variant Is Caused by a New Allele of Flower Color Gene W1 in Wild Soybean (Glycine soja)

Sundaramoorthy, Jagadeesh; Park, Gyu-Tae; Jo, Hyun; Lee, Jeong-Dong; Seo, Hak-Soo; Song, Jong-Tae

Abstract

The enzyme flavonoid 3',5'-hydroxylase (F3'5'H) plays an important role in producing anthocyanin pigments in soybean. Loss of function of the W1 locus encoding F3'5'H always produces white flowers. However, few color variations have been reported in wild soybean. In the present study, we isolated a new color variant of wild soybean accession (IT261811) with pinkish-white flowers. We found that the flower's pinkish-white color is caused by w1-s3, a single recessive allele of W1. The SNP detected in the mutant caused amino acid substitution (A(304)S) in a highly conserved SRS4 domain of F3'5'H proteins. On the basis of the results of the protein variation effect analyzer (PROVEAN) tool, we suggest that this mutation may lead to hypofunctional F3'5'H activity rather than non-functional activity, which thereby results in its pinkish-white color.

Keywords

flavonoid 3 ',5 '-hydroxylase; Glycine soja; pinkish-white flower; soybean; W1 locus

Published in

Agronomy
2021, volume: 11, number: 5, article number: 1001
Publisher: MDPI

Authors' information

Swedish University of Agricultural Sciences, Department of Plant Breeding
Kyungpook National University
Park, Gyu-Tae
Kyungpook National University
Jo, Hyun
Kyungpook National University
Lee, Jeong-Dong
Kyungpook National University
Seo, Hak-Soo
Seoul National University (SNU)
Song, Jong-Tae
Kyungpook National University

UKÄ Subject classification

Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.3390/agronomy11051001

URI (permanent link to this page)

https://res.slu.se/id/publ/112135