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Research article - Peer-reviewed, 2020

Reduction of Bacteria in Relation to Feeding Regimes When Treating Aquaculture Waste in Fly Larvae Composting

Lopes, Iva Guidini; Lalander, Cecilia; Vidotti, Rose Meire; Vinneras, Bjorn;

Abstract

This study evaluated the impact of feeding regimes on process performance and inactivation of microorganisms during treatment of aquaculture waste with black soldier fly (BSF) larvae. In three treatments (T1-T3), a blend of reclaimed bread and aquaculture waste was used as substrate for BSF larvae. In T1, the substrate was inoculated with four subtypes ofSalmonellaspp. andEscherichia coli(both at 1%w/w), and offered only once, at the beginning of the 14-day trial. In T2 and T3, the substrate was supplied on three different days, with contaminated substrate provided only the first event in T2 and in all three events in T3. Provision of a lump sum feeding (T1) proved unfavorable for larval growth and process efficiency, but did not affect the microbial reduction effect. The total reduction inSalmonellaspp. was approximately 6 log(10)in T1 and T2, and 3.3 log(10)in T3, while the total reduction inE. coliwas approximately 4 log(10)in T1 and T2, and 1.9 log(10)in T3. After removing the larvae, the treatment residues were re-inoculated withSalmonellaspp. andE. coli. It was found that the inactivation in both organisms continued in all treatments that originally contained BSF larvae (T1-T3), suggesting that antimicrobial substances may have been secreted by BSF larvae or by its associated microbiota.

Keywords

BSF; ecotechnology; hygienization; Escherichia coli; Salmonella Senftenberg; S; Typhimurium; S; Typhi; S; Dublin

Published in

Frontiers in Microbiology

2020, volume: 11, article number: 1616
Publisher: FRONTIERS MEDIA SA

Authors' information

Swedish University of Agricultural Sciences, Department of Energy and Technology
Sao Paulo State University
Swedish University of Agricultural Sciences, Department of Energy and Technology
Vidotti, Rose Meire
Sao Paulo State Univ UNESP
Swedish University of Agricultural Sciences, Department of Energy and Technology

UKÄ Subject classification

Microbiology

Publication Identifiers

DOI: https://doi.org/10.3389/fmicb.2020.01616

URI (permanent link to this page)

https://res.slu.se/id/publ/112324