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Research article - Peer-reviewed, 2020

Spruce sugars and poultry hydrolysate as growth medium in repeated fed-batch fermentation processes for production of yeast biomass

Lapena, David; Olsen, Pernille M.; Arntzen, Magnus O.; Kosa, Gergely; Passoth, Volkmar; Eijsink, Vincent G. H.; Horn, Svein J.


The production of microbial protein in the form of yeast grown on lignocellulosic sugars and nitrogen-rich industrial residues is an attractive approach for reducing dependency on animal and plant protein. Growth media composed of enzymatically saccharified sulfite-pulped spruce wood, enzymatic hydrolysates of poultry by-products and urea were used for the production of single-cell protein. Strains of three different yeast species, Cyberlindnera jadinii, Wickerhamomyces anomalus and Blastobotrys adeninivorans, were cultivated aerobically using repeated fed-batch fermentation up to 25 L scale. Wickerhamomyces anomalus was the most efficient yeast with yields of 0.6 g of cell dry weight and 0.3 g of protein per gram of glucose, with cell and protein productivities of 3.92 g/L/h and 1.87 g/L/h, respectively. Using the conditions developed here for producing W. anomalus, it would take 25 industrial (200 m(3)) continuously operated fermenters to replace 10% of the fish feed protein used in Norway.


Protein hydrolysate; Single-cell protein; Aquaculture; Downstream processing; Repeated batch

Published in

Bioprocess and Biosystems Engineering
2020, volume: 43, number: 4, pages: 723-736
Publisher: SPRINGER

Authors' information

Lapena, David
Norwegian University of Life Sciences
Olsen, Pernille M.
Norwegian University of Life Sciences
Arntzen, Magnus O.
Norwegian University of Life Sciences
Kosa, Gergely
Norwegian University of Life Sciences
Swedish University of Agricultural Sciences, Department of Molecular Sciences
Eijsink, Vincent G. H.
Norwegian University of Life Sciences
Horn, Svein J.
Norwegian University of Life Sciences

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