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Research article2020Peer reviewedOpen access

Spruce sugars and poultry hydrolysate as growth medium in repeated fed-batch fermentation processes for production of yeast biomass

Lapena, David; Olsen, Pernille M.; Arntzen, Magnus O.; Kosa, Gergely; Passoth, Volkmar; Eijsink, Vincent G. H.; Horn, Svein J.


The production of microbial protein in the form of yeast grown on lignocellulosic sugars and nitrogen-rich industrial residues is an attractive approach for reducing dependency on animal and plant protein. Growth media composed of enzymatically saccharified sulfite-pulped spruce wood, enzymatic hydrolysates of poultry by-products and urea were used for the production of single-cell protein. Strains of three different yeast species, Cyberlindnera jadinii, Wickerhamomyces anomalus and Blastobotrys adeninivorans, were cultivated aerobically using repeated fed-batch fermentation up to 25 L scale. Wickerhamomyces anomalus was the most efficient yeast with yields of 0.6 g of cell dry weight and 0.3 g of protein per gram of glucose, with cell and protein productivities of 3.92 g/L/h and 1.87 g/L/h, respectively. Using the conditions developed here for producing W. anomalus, it would take 25 industrial (200 m(3)) continuously operated fermenters to replace 10% of the fish feed protein used in Norway.


Protein hydrolysate; Single-cell protein; Aquaculture; Downstream processing; Repeated batch

Published in

Bioprocess and Biosystems Engineering
2020, Volume: 43, number: 4, pages: 723-736
Publisher: SPRINGER

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