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Review article - Peer-reviewed, 2021

Spray-induced gene silencing: an innovative strategy for plant trait improvement and disease control

Ramesh, Vetukuri; Dubey, Mukesh; Kalyandurg, Pruthvi Balachandra; Carlsson, Anders S.; Whisson, Stephen C.; Ortiz Rios, Rodomiro Octavio

Abstract

Modern plant breeding is still a time-consuming and costly process, even with the most advanced technologies such as gene editing. Hence, there is an urgent need to develop alternative means for plant trait manipulation and plant protection. RNA interference (RNAi) is a conserved cellular mechanism mediated by naturally occurring double-stranded RNA (dsRNA) and small RNAs (sRNAs) that can target mRNAs for destruction or transcript reduction. Here, we review the potential of technology based on RNAi, called spray-induced gene silencing (SIGS), as an alternative or adjunct to breeding for manipulation of endogenous gene expression in plants or pathogen control. SIGS based on exogenous application of RNA molecules in plants may be especially useful in reducing pest or pathogen impacts, thereby ameliorating biotic stresses and increasing the agronomic performance of crops.

Keywords

RNA interference; small RNAs; SIGS; dsRNAs

Published in

Crop breeding and applied biotechnology
2021, volume: 21, article number: e387921S11

Authors' information

Swedish University of Agricultural Sciences, Department of Plant Breeding
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Swedish University of Agricultural Sciences, Department of Plant Breeding
Carlsson, Anders S. (Carlsson, Anders)
Swedish University of Agricultural Sciences, Department of Plant Breeding
Whisson, Stephen C.
The James Hutton Institute
Swedish University of Agricultural Sciences, Department of Plant Breeding

UKÄ Subject classification

Genetics and Breeding

Publication Identifiers

DOI: https://doi.org/10.1590/1984-70332021v21Sa24

URI (permanent link to this page)

https://res.slu.se/id/publ/112628