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Research article - Peer-reviewed, 2021

Prevalence of Orthohantavirus-Reactive Antibodies in Humans and Peri-Domestic Rodents in Northern Ethiopia

Meheretu, Yonas; Granberg, Asa; Berhane, Gebregiorgis; Khalil, Hussein; Lwande, Olivia Wesula; Mitiku, Mengistu; Welegerima, Kiros; de Bellocq, Joelle Gouy; Bryja, Josef; Abreha, Hagos; Leirs, Herwig; Ecke, Frauke; Evander, Magnus


In 2012, Tigray orthohantavirus was discovered in Ethiopia, but its seasonal infection in small mammals, and whether it poses a risk to humans was unknown. The occurrence of small mammals, rodents and shrews, in human inhabitations in northern Ethiopia is affected by season and presence of stone bunds. We sampled small mammals in two seasons from low- and high-density stone bund fields adjacent to houses and community-protected semi-natural habitats in Atsbi and Hagere Selam, where Tigray orthohantavirus was first discovered. We collected blood samples from both small mammals and residents using filter paper. The presence of orthohantavirus-reactive antibodies in blood was then analyzed using immunofluorescence assay (human samples) and enzyme linked immunosorbent assays (small mammal samples) with Puumala orthohantavirus as antigen. Viral RNA was detected by RT-PCR using small mammal blood samples. Total orthohantavirus prevalence (antibodies or virus RNA) in the small mammals was 3.37%. The positive animals were three Stenocephalemys albipes rats (prevalence in this species = 13.04%). The low prevalence made it impossible to determine whether season and stone bunds were associated with orthohantavirus prevalence in the small mammals. In humans, we report the first detection of orthohantavirus-reactive IgG antibodies in Ethiopia (seroprevalence = 5.26%). S. albipes lives in close proximity to humans, likely increasing the risk of zoonotic transmission.


orthohantavirus; rodents; rural community; risk factors; Ethiopia

Published in

2021, volume: 13, number: 6, article number: 1054
Publisher: MDPI

Authors' information

Mekelle University
Granberg, Asa
No organisation
Berhane, Gebregiorgis
Mekelle University
Swedish University of Agricultural Sciences, Department of Wildlife, Fish and Environmental Studies
Lwande, Olivia Wesula
Umeå University
Mitiku, Mengistu
Mekelle University
Welegerima, Kiros
Mekelle University
de Bellocq, Joelle Gouy
Czech University of Life Sciences Prague
Bryja, Josef
Institute of Vertebrate Biology of the Czech Academy of Sciences
Abreha, Hagos
Mekelle University
Leirs, Herwig
University of Antwerp
Swedish University of Agricultural Sciences, Department of Wildlife, Fish and Environmental Studies
Evander, Magnus
Umea University

UKÄ Subject classification

Microbiology in the medical area

Publication Identifiers


URI (permanent link to this page)