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Research article - Peer-reviewed, 2021

Pyramiding of scald resistance genes in four spring barley MAGIC populations

Hautsalo, Juho; Novakazi, Fluture; Jalli, Marja; Goransson, Magnus; Manninen, Outi; Isolahti, Mika; Reitan, Lars; Bergersen, Stein; Krusell, Lene; Robertsen, Charlotte Damsgard; Orabi, Jihad; Jensen, Jens Due; Jahoor, Ahmed; Bengtsson, Therese

Abstract

Genome-Wide Association Studies (GWAS) of four Multi-parent Advanced Generation Inter-Cross (MAGIC) populations identified nine regions on chromosomes 1H, 3H, 4H, 5H, 6H and 7H associated with resistance against barley scald disease. Three of these regions are putatively novel resistance Quantitative Trait Loci (QTL). Barley scald is caused by Rhynchosporium commune, one of the most important barley leaf diseases that are prevalent in most barley-growing regions. Up to 40% yield losses can occur in susceptible barley cultivars. Four MAGIC populations were generated in a Nordic Public-Private Pre-breeding of spring barley project (PPP Barley) to introduce resistance to several important diseases. Here, these MAGIC populations consisting of six to eight founders each were tested for scald resistance in field trials in Finland and Iceland. Eight different model covariate combinations were compared for GWAS studies, and the models that deviated the least from the expected p-values were selected. For all QTL, candidate genes were identified that are predicted to be involved in pathogen defence. The MAGIC progenies contained new haplotypes of significant SNP-markers with high resistance levels. The lines with successfully pyramided resistance against scald and mildew and the significant markers are now distributed among Nordic plant breeders and will benefit development of disease-resistant cultivars.

Keywords

Rhynchosporium commune; GWAS; Hordeum vulgare L; Multi-parent Advanced Generation Inter-Cross; FarmCPU; BLINK

Published in

TAG Theoretical and Applied Genetics
2021, volume: 134, pages: 3829-3843
Publisher: SPRINGER

Authors' information

Hautsalo, Juho
Natural Resources Institute Finland (LUKE)
Swedish University of Agricultural Sciences, Department of Plant Breeding
Jalli, Marja
Natural Resources Institute Finland (LUKE)
Göransson, Magnus
Agricultural University of Iceland (LBHI)
Manninen, Outi
Boreal Plant Breeding Ltd
Isolahti, Mika
Boreal Plant Breeding Ltd
Bergersen, Stein
Graminor
Reitan, Lars
Graminor
Krusell, Lene
Sejet Plant Breeding
Damsgård Robertsen, Charlotte
Sejet Plant Breeding
Orabi, Jihad
Nordic Seed A/S
Jensen, Jens Due
Nordic Seed A/S
Nordic Seed A/S
Swedish University of Agricultural Sciences, Department of Plant Breeding
Swedish University of Agricultural Sciences, Department of Plant Breeding
Veteläinen, Merja
No organisation
Alsheikh, Muath
No organisation

UKÄ Subject classification

Genetics and Breeding

Publication Identifiers

DOI: https://doi.org/10.1007/s00122-021-03930-y

URI (permanent link to this page)

https://res.slu.se/id/publ/112855