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Research article - Peer-reviewed, 2021

Isolation and modification of nano-scale cellulose from organosolv-treated birch through the synergistic activity of LPMO and endoglucanases

Muraleedharan, Madhu Nair; Karnaouri, Anthi; Piatkova, Maria; Ruiz-Caldas, Maria-Ximena; Matsakas, Leonidas; Liu, Bing; Rova, Ulrika; Christakopoulos, Paul; Mathew, Aji P.

Abstract

Nanocellulose isolation fromlignocellulose is a tedious and expensive processwith high energy and harsh chemical requirements, primarily due to the recalcitrance of the substrate, which otherwise would have been costeffective due to its abundance. Replacing the chemical steps with biocatalytic processes offers opportunities to solve this bottleneck to a certain extent due to the enzymes substrate specificity and mild reaction chemistry. In this work, we demonstrate the isolation of sulphate-free nanocellulose from organosolv pretreated birch biomass using different glycosyl-hydrolases, along with accessory oxidative enzymes including a lytic polysaccharide monooxygenase (LPMO). The suggested process produced colloidal nanocellulose suspensions (zeta-potential-19.4 mV) with particles of 7-20 nm diameter, high carboxylate content and improved thermostability (T-o= 301 degrees C, T-max= 337 degrees C). Nanocelluloseswere subjected to post-modification using LPMOs of different regioselectivity. The sample from chemical route was the least favorable for LPMO to enhance the carboxylate content, while that from the C1-specific LPMO treatment showed the highest increase in carboxylate content. (c) 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).

Keywords

Nanocellulose; LPMO biocatalysis; Post-treatment modification/functionalization

Published in

International Journal of Biological Macromolecules
2021, volume: 183, pages: 101-109
Publisher: ELSEVIER

Authors' information

Muraleedharan, Madhu Nair
Lulea University of Technology
Karnaouri, Anthi
Lulea University of Technology
Piatkova, Maria
Stockholm University
Ruiz-Caldas, Maria-Ximena
Stockholm University
Matsakas, Leonidas
Lulea University of Technology
Liu, Bing
Swedish University of Agricultural Sciences, Department of Molecular Sciences
Rova, Ulrika
Lulea University of Technology
Christakopoulos, Paul
Lulea University of Technology
Mathew, Aji P.
Stockholm University

UKÄ Subject classification

Biocatalysis and Enzyme Technology
Nano-technology

Publication Identifiers

DOI: https://doi.org/10.1016/j.ijbiomac.2021.04.136

URI (permanent link to this page)

https://res.slu.se/id/publ/113219