Research article - Peer-reviewed, 2021
Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in ArabidopsisGutierrez-Beltran, Emilio; Elander, Pernilla H.; Dalman, Kerstin; Dayhoff, Guy W.; Moschou, Panagiotis N.; Uversky, Vladimir N.; Crespo, Jose L.; Bozhkov, Peter, V
AbstractTudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plant-specific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein-protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signalling.
Keywordsheat stress; intrinsically disordered regions; SnRK1; SNF1; AMPK; stress granules; tudor staphylococcal nuclease
Published inThe Embo Journal
2021, volume: 40, number: 17, article number: e105043
University of Sevilla
Dayhoff, Guy W.
University of South Florida
Foundation for Research and Technology - Hellas (FORTH)
University of Crete
Swedish University of Agricultural Sciences, Department of Plant Biology
Uversky, Vladimir N.
Russian Academy of Sciences
Crespo, Jose L.
University of Sevilla
UKÄ Subject classification
Biochemistry and Molecular Biology
URI (permanent link to this page)