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Research article - Peer-reviewed, 2021

Pathogen-Mediated Stomatal Opening: A Previously Overlooked Pathogenicity Strategy in the Oomycete Pathogen Phytophthora infestans

Yang, Li-Na; Liu, Hao; Wang, Yan-Ping; Seematti, Jenifer; Grenville-Briggs, Laura J. J.; Wang, Zonghua; Zhan, Jiasui

Abstract

Phytophthora infestans, the most damaging oomycete pathogen of potato, is specialized to grow sporangiophore through opened stomata for secondary inoculum production. However, it is still unclear which metabolic pathways in potato are manipulated by P. infestans in the guard cell-pathogen interactions to open the stomata. Here microscopic observations and cell biology were used to investigate antagonistic interactions between guard cells and the oomycete pathogen. We observed that the antagonistic interactions started at the very beginning of infection. Stomatal movement is an important part of the immune response of potato to P. infestans infection and this occurs through guard cell death and stomatal closure. We observed that P. infestans appeared to manipulate metabolic processes in guard cells, such as triacylglycerol (TAG) breakdown, starch degradation, H2O2 scavenging, and NO catabolism, which are involved in stomatal movement, to evade these stomatal defense responses. The signal transduction pathway of P. infestans-induced stomatal opening likely starts from H2O2 and NO scavenging, along with TAG breakdown while the subsequent starch degradation reinforces the opening process by strengthening guard cell turgor and opening the stomata to their maximum aperture. These results suggest that stomata are a barrier stopping P. infestans from completing its life cycle, but this host defense system can be bypassed through the manipulation of diverse metabolic pathways that may be induced by P. infestans effector proteins.

Keywords

stomatal immunity; starch degradation; triacylglycerol breakdown; phytophthora infestans; potato defences

Published in

Frontiers in Plant Science
2021, volume: 12, article number: 668797
Publisher: FRONTIERS MEDIA SA

Authors' information

Yang, Li-Na
Minjiang University
Liu, Hao
Fujian Agriculture and Forestry University
Wang, Yan-Ping
Fujian Agriculture and Forestry University
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Wang, Zonghua
Minjiang University
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology

UKÄ Subject classification

Agricultural Science
Cell Biology

Publication Identifiers

DOI: https://doi.org/10.3389/fpls.2021.668797

URI (permanent link to this page)

https://res.slu.se/id/publ/113302