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Research article - Peer-reviewed, 2021

Long-term maize-Desmodium intercropping shifts structure and composition of soil microbiome with stronger impact on fungal communities

Mwakilili, Aneth David; Mwaikono, Kilaza Samson; Herrera, Sebastian Larsson; Midega, Charles A. O.; Magingo, Francis; Alsanius, Beatrix; Dekker, Teun; Lyantagaye, Sylvester Leonard

Abstract

Purpose Push-pull is an intercropping technology that is rapidly spreading among smallholder farmers in Sub-Saharan Africa. The technology intercrops cereals with Desmodium to fight off stem borers, eliminate parasitic weeds, and improve soil fertility and yields of cereals. The above-ground components of push-pull cropping have been well investigated. However, the impact of the technology on the soil microbiome and the subsequent role of the microbiome on diverse ecosystem benefits are unknown. Here we describe the soil microbiome associated with maize-Desmodium intercropping in push-pull farming in comparison to long-term maize monoculture. Methods Soil samples were collected from long-term maize-Desmodium intercropping and maize monoculture plots at the international centre for insect physiology and ecology (ICIPE), Mbita, Kenya. Total DNA was extracted before16S rDNA and ITS sequencing and subsequent analysis on QIIME2 and R. Results Maize-Desmodium intercropping caused a strong divergence in the fungal microbiome, which was more diverse and species rich than monoculture plots. Fungal groups enriched in intercropping plots are linked to important ecosystem services, belonging to functional groups such as mycorrhiza, endophytes, saprophytes, decomposers and bioprotective fungi. Fewer fungal genera were enriched in monoculture plots, some of which were associated with plant pathogenesis and opportunistic infection in humans. In contrast, the impact of intercropping on soil bacterial communities was weak with few differences between intercropping and monoculture. Conclusion Maize-Desmodium intercropping diversifies fungal microbiomes and favors taxa associated with important ecosystem services including plant health, productivity and food safety.

Keywords

Soil microbiome; Push-pull farming; Intercropping microbiome; 16S; ITS; Desmodium spp

Published in

Plant and Soil
2021,
Publisher: SPRINGER

Authors' information

David Mwakilili, Aneth (Bella David, Aneth)
University of Dar es Salaam
David Mwakilili, Aneth (Bella David, Aneth)
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Midega, Charles A. O.
North West University - South Africa
Magingo, Francis
University of Dar es Salaam
Swedish University of Agricultural Sciences, Department of Biosystems and Technology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Lyantagaye, Sylvester Leonard
University of Dar es Salaam

Sustainable Development Goals

SDG15 Life on land
SDG2 Zero hunger

UKÄ Subject classification

Soil Science

Publication Identifiers

DOI: https://doi.org/10.1007/s11104-021-05082-w

URI (permanent link to this page)

https://res.slu.se/id/publ/113308