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Research article - Peer-reviewed, 2021

A fast, nondestructive method for the detection of disease-related lesions and wounded leaves

Zahid, Muhammad Awais; Sandroni, Murilo; Vetukuri, Ramesh Raju; Andreasson, Erik

Abstract

Trypan blue staining is a classic way of visualizing leaf disease and wound responses in plants, but it involves working with toxic chemicals and is time-consuming (2-3 days). Here, the investigators established near-infrared scanning with standard lab equipment as a fast and nondestructive method for the analysis of leaf injuries compared with trypan blue staining. Pathogen-inoculated and wounded leaves from potato, tomato, spinach, strawberry, and arabidopsis plants were used for proof of concept. The results showed that this newly developed protocol with near-infrared scanning gave the same results as trypan blue staining. Furthermore, a macro in FIJI was made to quantify the leaf damage. The new protocol was time-efficient, nondestructive, chemical-free and may be used for high-throughput studies.

Keywords

image processing; leaf damage; lesions; phenotyping; plant disease; potato; Phytophthora

Published in

Biotechniques
2021, volume: 71, number: 2
Publisher: FUTURE SCI LTD

Authors' information

Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Vetukuri, Ramesh (Ramesh, Vetukuri)
Swedish University of Agricultural Sciences, Department of Plant Breeding
Swedish University of Agricultural Sciences, Department of Plant Protection Biology

UKÄ Subject classification

Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.2144/btn-2021-0045

URI (permanent link to this page)

https://res.slu.se/id/publ/113434