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Review article - Peer-reviewed, 2021

RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture

Rajput, Meenakshi; Choudhary, Khushboo; Kumar, Manish; Vivekanand, V.; Chawade, Aakash; Ortiz, Rodomiro; Pareek, Nidhi


With the rapid population growth, there is an urgent need for innovative crop improvement approaches to meet the increasing demand for food. Classical crop improvement approaches involve, however, a backbreaking process that cannot equipoise with increasing crop demand. RNA-based approaches i.e., RNAi-mediated gene regulation and the site-specific nuclease-based CRISPR/Cas9 system for gene editing has made advances in the efficient targeted modification in many crops for the higher yield and resistance to diseases and different stresses. In functional genomics, RNA interference (RNAi) is a propitious gene regulatory approach that plays a significant role in crop improvement by permitting the downregulation of gene expression by small molecules of interfering RNA without affecting the expression of other genes. Gene editing technologies viz. the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (CRISPR/Cas) have appeared prominently as a powerful tool for precise targeted modification of nearly all crops’ genome sequences to generate variation and accelerate breeding efforts. In this regard, the review highlights the diverse roles and applications of RNAi and CRISPR/Cas9 system as powerful technologies to improve agronomically important plants to enhance crop yields and increase tolerance to environmental stress (biotic or abiotic). Ultimately, these technologies can prove to be important in view of global food security and sustainable agriculture.


crop; CRISPR/Cas9; resistance; RNA interference; stress

Published in

2021, volume: 10, number: 9, article number: 1914

Authors' information

Rajput, Meenakshi
Central University of Rajasthan (CURAJ)
Choudhary, Khushboo
Central University of Rajasthan (CURAJ)
Kumar, Manish
Central University of Rajasthan
Vivekanand, V.
Malaviya National Institute of Technology Jaipur (MNIT)
Swedish University of Agricultural Sciences, Department of Plant Breeding
Ortiz, Rodomiro (Ortiz Rios, Rodomiro Octavio)
Swedish University of Agricultural Sciences, Department of Plant Breeding
Pareek, Nidhi
Central University of Rajasthan

Sustainable Development Goals

SDG2 Zero hunger

UKÄ Subject classification

Plant Biotechnology

Publication Identifiers


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