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Research article - Peer-reviewed, 2021

Spatio-temporal distribution of DMI and SDHI fungicide resistance of Zymoseptoria tritici throughout Europe based on frequencies of key target-site alterations

Hellin, Pierre; Duvivier, Maxime; Heick, Thies M.; Fraaije, Bart A.; Bataille, Charlotte; Clinckemaillie, Aurelie; Legreve, Anne; Jorgensen, Lise N.; Andersson, Bjorn; Samils, Berit; Rodemann, Bernd; Berg, Gunilla; Hutton, Fiona; Garnault, Maxime; El Jarroudi, Moussa; Couleaud, Gilles; Kildea, Steven


BACKGROUND Over the past decade, demethylation inhibitor (DMI) and succinate dehydrogenase inhibitor (SDHI) fungicides have been extensively used to control to septoria tritici blotch, caused by Zymoseptoria tritici on wheat. This has led to the development and selection of alterations in the target-site enzymes (CYP51 and SDH, respectively). RESULTS Taking advantage of newly and previously developed qPCR assays, the frequency of key alterations associated with DMI (CYP51-S524T) and SDHI (SDHC-T79N/I, C-N86S and C-H152R) resistance was assessed in Z. tritici-infected wheat leaf samples collected from commercial crops (n = 140) across 14 European countries prior to fungicide application in the spring of 2019. This revealed the presence of a West to East gradient in the frequencies of the most common key alterations conferring azole (S524T) and SDHI resistance (T79N and N86S), with the highest frequencies measured in Ireland and Great Britain. These observations were corroborated by sequencing (CYP51 and SDH subunits) and sensitivity phenotyping (prothioconazole-desthio and fluxapyroxad) of Z. tritici isolates collected from a selection of field samples. Additional sampling made at the end of the 2019 season confirmed the continued increase in frequency of the targeted alterations. Investigations on historical leaf DNA samples originating from different European countries revealed that the frequency of all key alterations (except C-T79I) has been gradually increasing over the past decade. CONCLUSION Whilst these alterations are quickly becoming dominant in Ireland and Great Britain, scope still exists to delay their selection throughout the wider European population, emphasizing the need for the implementation of fungicide antiresistance measures.


Mycosphaerella graminicola; sensitivity; disease control; septoria tritici blotch; triazole; wheat

Published in

Pest Management Science
2021, volume: 77, number: 12, pages: 5576-5588

Authors' information

Hellin, Pierre
Walloon Agricultural Research Centre
Duvivier, Maxime
Walloon Agricultural Research Centre
Heick, Thies M.
Aarhus University
Fraaije, Bart A.
National Insititute of Agricultural Botany (NIAB)
Bataille, Charlotte
Walloon Agricultural Research Centre
Clinckemaillie, Aurélie
Walloon Agricultural Research Centre
Legreve, Anne
Universite Catholique Louvain
Jorgensen, Lise N.
Aarhus University
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Rodemann, Bernd
Julius Kuhn-Institut
Berg, Gunilla
Swedish Board of Agriculture
Hutton, Fiona
Garnault, Maxime
Universite Paris Saclay
El Jarroudi, Moussa
University of Liege
Couleaud, Gilles
ARVALIS Institut du Végétal
Kildea, Steven

Associated SLU-program

SLU Network Plant Protection

UKÄ Subject classification

Agricultural Science

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