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Research article - Peer-reviewed, 2021

Characterization of polycyclic aromatic compounds in historically contaminated soil by targeted and non-targeted chemical analysis combined with in vitro bioassay

Titaley, Ivan A.; Lam, Monika M.; Bulow, Rebecca; Enell, Anja; Wiberg, Karin; Larsson, Maria

Abstract

Soil samples from a contaminated site in Sweden were analyzed to identify the presence of 78 polycyclic aromatic compounds (PACs) using gas chromatography coupled with mass spectrometry (GC-MS). The target analysis revealed large contributions not only from polycyclic aromatic hydrocarbons (PAHs), but also from alkylated- and oxygenated-PAHs (alkyl- and oxy-PAHs, respectively), and N-heterocyclics (NPACs). PAC profiles indicated primarily pyrogenic sources, although contribution of petrogenic sources was also observed in one sample as indicated by a high ratio of alkylated naphthalene compared to naphthalene. The aryl hydrocarbon receptor (AhR)-activity of the soil extracts was assessed using the H4IIe-pGudluc 1.1 cells bioassay. When compared with the calculated total AhR-activity of the PACs in the target list, 35-97% of the observed bioassay activity could be explained by 62 PACs with relative potency factors (REPs). The samples were further screened using GC coupled with OrbitrapTM high resolution MS (GC-HRMS) to investigate the presence of other PACs that could potentially contribute to the AhR-activity of the extracts. 114 unique candidate compounds were tentatively identified and divided into four groups based on their AhR-activity and environmental occurrence. Twelve substances satisfied all the criteria, and these compounds are suggested to be included in regular screening in future studies, although their identities were not confirmed by standards in this study. High unexplained bio-TEQ fractions in three of the samples may be explained by tentatively identified compounds (n = 35) with high potential of being toxic. This study demonstrates the benefit of combining targeted and non-targeted chemical analysis with bioassay analysis to assess the diversity and effects of PACs at contaminated sites. The applied prioritization strategy revealed a number of tentatively identified compounds, which likely contributed to the overall bioactivity of the soil extracts.

Keywords

Polycyclic aromatic compounds; Orbitrap(TM )GC-MS; Full-scan screening; Non-targeted analysis; Aryl hydrocarbon receptor-activity; Bio-TEQ

Published in

Environmental Pollution
2021, volume: 289, article number: 117910
Publisher: ELSEVIER SCI LTD

Authors' information

Titaley, Ivan A.
Örebro University
Lam, Monika M.
Örebro University
Bülow, Rebecca
Örebro University
Enell, Anja
Swedish Geotechnical Institute (SGI)
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment
Larsson, Maria
Örebro University

UKÄ Subject classification

Environmental Sciences

Publication Identifiers

DOI: https://doi.org/10.1016/j.envpol.2021.117910

URI (permanent link to this page)

https://res.slu.se/id/publ/113752