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Research article - Peer-reviewed, 2021

The radish Ogura fertility restorer impedes translation elongation along its cognate CMS-causing mRNA

Wang, Chuande; Lezhneva, Lina; Arnal, Nadege; Quadrado, Martine; Mireau, Hakim

Abstract

The control of messenger RNA (mRNA) translation has been increasingly recognized as a key regulatory step for gene control, but clear examples in eukaryotes are still scarce. Nucleo-cytoplasmic male sterilities (CMS) represent ideal genetic models to dissect genetic interactions between the mitochondria and the nucleus in plants. This trait is determined by specific mitochondrial genes and is associated with a pollen sterility phenotype that can be suppressed by nuclear genes known as restorer-of-fertility (Rf). In this study, we focused on the Ogura CMS system in rapeseed and showed that reversion to male sterility by the PPR-B fertility restorer (also called Rfo) occurs through a specific translation inhibition of the mitochondria-encoded CMS causing mRNA orf138. We also demonstrate that PPR-B binds within the coding sequence of orf138 and acts as a ribosome blocker to specifically impede translation elongation along the orf138 mRNA. Rfo is the first recognized fertility restorer shown to act this way. These observations will certainly facilitate the development of synthetic fertility restorers for CMS systems in which efficient natural Rfs are lacking.

Keywords

cytoplasmic male sterility; restorer-of-fertility; translation; mitochondria; pentatricopeptide repeat

Published in

Proceedings of the National Academy of Sciences of the United States of America
2021, volume: 118, number: 35, article number: e2105274118
Publisher: NATL ACAD SCIENCES

Authors' information

Wang, Chuande
Universite Paris Saclay
Lezhneva, Lina
University Paris-Saclay
Arnal, Nadege
Universite Paris Saclay
Quadrado, Martine
Universite Paris Saclay
Mireau, Hakim
Universite Paris Saclay

UKÄ Subject classification

Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.1073/pnas.2105274118

URI (permanent link to this page)

https://res.slu.se/id/publ/113800