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Research article - Peer-reviewed, 2021

Novel Microdialysis Technique Reveals a Dramatic Shift in Metabolite Secretion during the Early Stages of the Interaction between the Ectomycorrhizal Fungus Pisolithus microcarpus and Its Host Eucalyptus grandis

Plett, Krista L.; Buckley, Scott; Plett, Jonathan M.; Anderson, Ian C.; Lundberg-Felten, Judith; Jamtgard, Sandra

Abstract

The colonisation of tree roots by ectomycorrhizal (ECM) fungi is the result of numerous signalling exchanges between organisms, many of which occur before physical contact. However, information is lacking about these exchanges and the compounds that are secreted by each organism before contact. This is in part due to a lack of low disturbance sampling methods with sufficient temporal and spatial resolution to capture these exchanges. Using a novel in situ microdialysis approach, we sampled metabolites released from Eucalyptus grandis and Pisolithus microcarpus independently and during indirect contact over a 48-h time-course using UPLC-MS. A total of 560 and 1530 molecular features (MFs; ESI- and ESI+ respectively) were identified with significant differential abundance from control treatments. We observed that indirect contact between organisms altered the secretion of MFs to produce a distinct metabolomic profile compared to either organism independently. Many of these MFs were produced within the first hour of contact and included several phenylpropanoids, fatty acids and organic acids. These findings show that the secreted metabolome, particularly of the ECM fungus, can rapidly shift during the early stages of pre-symbiotic contact and highlight the importance of observing these early interactions in greater detail. We present microdialysis as a useful tool for examining plant-fungal signalling with high temporal resolution and with minimal experimental disturbance.

Keywords

metabolomics; pre-symbiosis signalling; ectomycorrhizal fungi; microdialysis

Published in

Microorganisms
2021, volume: 9, number: 9, article number: 1817
Publisher: MDPI

Authors' information

Plett, Krista L.
NSW Department of Primary Industries
Plett, Krista L.
Western Sydney University
Swedish University of Agricultural Sciences, Department of Forest Ecology and Management
Plett, Jonathan M.
Western Sydney University
Anderson, Ian C.
Western Sydney University
Swedish University of Agricultural Sciences, Department of Forest Ecology and Management

UKÄ Subject classification

Microbiology

Publication Identifiers

DOI: https://doi.org/10.3390/microorganisms9091817

URI (permanent link to this page)

https://res.slu.se/id/publ/113898