Review article - Peer-reviewed, 2021
Current Insight into Culture-Dependent and Culture-Independent Methods in Discovering Ascomycetous TaxaWijayawardene, Nalin N.; Bahram, Mohammad; Sanchez-Castro, Ivan; Dai, Dong-Qin; Ariyawansa, Kahandawa G. S. U.; Jayalal, Udeni; Suwannarach, Nakarin; Tedersoo, Leho
AbstractCulture techniques are vital in both traditional and modern fungal taxonomy. Establishing sexual-asexual links and synanamorphs, extracting DNA and secondary metabolites are mainly based on cultures. However, it is widely accepted that a large number of species are not sporulating in nature while others cannot be cultured. Recent ecological studies based on culture-independent methods revealed these unculturable taxa, i.e., dark taxa. Recent fungal diversity estimation studies suggested that environmental sequencing plays a vital role in discovering missing species. However, Sanger sequencing is still the main approach in determining DNA sequences in culturable species. In this paper, we summarize culture-based and culture-independent methods in the study of ascomycetous taxa. High-throughput sequencing of leaf endophytes, leaf litter fungi and fungi in aquatic environments is important to determine dark taxa. Nevertheless, currently, naming dark taxa is not recognized by the ICN, thus provisional naming of them is essential as suggested by several studies.
Keywordsconsolidated species concept; fungal diversity; molecular taxonomy; morphological characters; traditional taxonomy; polyphasic approach
Published inJournal of Fungi
2021, volume: 7, number: 9, article number: 703
Wijayawardene, Nalin N.
Guizhou Medical University
University of Tartu
University of Granada
Qujing Normal University
Ariyawansa, Kahandawa G. S. U.
University of Colombo
Sabaragamuwa University of Sri Lanka
Chiang Mai University
King Saud University
University of Tartu
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