Research article - Peer-reviewed, 2021
Seminiferous epithelium cycle staging based on the development of the acrosome in ram testis
Sturm, Sabina; Svara, Tanja; Sporndly-Nees, Ellinor; Cerkvenik-Flajs, Vesna; Gombac, Mitja; Weber, Anna-Luisa; Weber, KlausAbstract
Testicular histopathology is considered the most sensitive and reliable method to detect the effects of chemicals on sperm production. To carry out a sensitive examination of testicular histopathology and interpret the changes require knowledge of spermatogenic stages. Spermatogenic staging based on acrosome development during spermiogenesis is conventionally performed in animal species routinely used for research and toxicity testing. In contrast, small ruminants, such as sheep and goats, are rarely used as animal models to evaluate toxicity in male reproductive organs. To the best of our knowledge, a comparable spermatogenic staging system in rams has not yet been fully characterised. Hence, this study aimed to adapt the existing spermatogenic staging based on acrosome development in bull testes to fit the seminiferous epithelium cycle of ram testes. The results show that spermatogenic staging based on acrosome development in bull testes can, with slight modifications, be efficiently used for the staging of ram testes.Keywords
ram; spermatogenesis; staging; acrosome development; spermiogenesisPublished in
Journal of toxicologic pathology2021, volume: 34, number: 4, pages: 331-338
Publisher: JAPANESE SOC TOXICOLOGIC PATHOLOGY
Authors' information
Sturm, Sabina
University of Ljubljana
Svara, Tanja
University of Ljubljana
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
Cerkvenik-Flajs, Vesna
University of Ljubljana
Gombac, Mitja
University of Ljubljana
Weber, Anna-Luisa
AnaPath Services
Weber, Klaus
AnaPath Serv GmbH
UKÄ Subject classification
Pathobiology
Publication Identifiers
DOI: https://doi.org/10.1293/tox.2021-0025
URI (permanent link to this page)
https://res.slu.se/id/publ/114268