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Research article - Peer-reviewed, 2021

Characterization of germline development and identification of genes associated with germline specification in pineapple

Zhao, Lihua; Liu, Liping; Liu, Yanhui; Dou, Xianying; Cai, Hanyang; Aslam, Mohammad; Hou, Zhimin; Jin, Xingyue; Li, Yi; Wang, Lulu; Zhao, Heming; Wang, Xiaomei; Sicard, Adrien; Qin, Yuan

Abstract

Understanding germline specification in plants could be advantageous for agricultural applications. In recent decades, substantial efforts have been made to understand germline specification in several plant species, including Arabidopsis, rice, and maize. However, our knowledge of germline specification in many agronomically important plant species remains obscure. Here, we characterized the female germline specification and subsequent female gametophyte development in pineapple using callose staining, cytological, and whole-mount immunolocalization analyses. We also determined the male germline specification and gametophyte developmental timeline and observed male meiotic behavior using chromosome spreading assays. Furthermore, we identified 229 genes that are preferentially expressed at the megaspore mother cell (MMC) stage during ovule development and 478 genes that are preferentially expressed at the pollen mother cell (PMC) stage of anther development using comparative transcriptomic analysis. The biological functions, associated regulatory pathways and expression patterns of these genes were also analyzed. Our study provides a convenient cytological reference for exploring pineapple germline development and a molecular basis for the future functional analysis of germline specification in related plant species.

Published in

Horticulture Research
2021, volume: 8, number: 1, article number: 239
Publisher: NANJING AGRICULTURAL UNIV

Authors' information

Swedish University of Agricultural Sciences, Department of Plant Biology
Fujian Agriculture and Forestry University
Liu, Liping
Fujian Agriculture and Forestry University
Liu, Yanhui
Fujian Agriculture and Forestry University
Dou, Xianying
Fujian Agriculture and Forestry University
Cai, Hanyang
Fujian Agriculture and Forestry University
Aslam, Mohammad
Guangxi University
Hou, Zhimin
Fujian Agriculture and Forestry University
Jin, Xingyue
Fujian Agriculture and Forestry University
Li, Yi
Fujian Agriculture and Forestry University
Wang, Lulu
Fujian Agriculture and Forestry University
Zhao, Heming
Fujian Agriculture and Forestry University
Wang, Xiaomei
Guangxi Academy of Agricultural Sciences
Sicard, Adrien (Sicard, Adrien)
Swedish University of Agricultural Sciences, Department of Plant Biology
Qin, Yuan
Guangxi University

UKÄ Subject classification

Horticulture

Publication Identifiers

DOI: https://doi.org/10.1038/s41438-021-00669-x

URI (permanent link to this page)

https://res.slu.se/id/publ/114293