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Research article - Peer-reviewed, 2022

Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis

Oliver, Cecilia; Martinez, German

Abstract

Meiosis is a specialized cell division that is key for reproduction and genetic diversity in sexually reproducing plants. Recently, different RNA silencing pathways have been proposed to carry a specific activity during meiosis, but the pathways involved during this process remain unclear. Here, we explored the subcellular localization of different ARGONAUTE (AGO) proteins, the main effectors of RNA silencing, during male meiosis in Arabidopsis thaliana using immunolocalizations with commercially available antibodies. We detected the presence of AGO proteins associated with posttranscriptional gene silencing (AGO1, 2, and 5) in the cytoplasm and the nucleus, while AGOs associated with transcriptional gene silencing (AGO4 and 9) localized exclusively in the nucleus. These results indicate that the localization of different AGOs correlates with their predicted roles at the transcriptional and posttranscriptional levels and provide an overview of their timing and potential role during meiosis.

Keywords

Meiosis; RNA silencing; Argonaute; Small RNAs; Pollen; Epigenetics

Published in

Plant Reproduction
2022, volume: 35, number: 2, pages: 153-160
Publisher: SPRINGER

Authors' information

Oliver Velasco, Cecilia
Swedish University of Agricultural Sciences, Department of Plant Biology
Martinez Arias, German (Martinez-Arias, Germán)
Swedish University of Agricultural Sciences, Department of Plant Biology

UKÄ Subject classification

Genetics

Publication Identifiers

DOI: https://doi.org/10.1007/s00497-021-00434-z

URI (permanent link to this page)

https://res.slu.se/id/publ/114528