Oliver Velasco, Cecilia
- Department of Plant Biology, Swedish University of Agricultural Sciences
Abstract
Meiosis is a specialized cell division that is key for reproduction and genetic diversity in sexually reproducing plants. Recently, different RNA silencing pathways have been proposed to carry a specific activity during meiosis, but the pathways involved during this process remain unclear. Here, we explored the subcellular localization of different ARGONAUTE (AGO) proteins, the main effectors of RNA silencing, during male meiosis in Arabidopsis thaliana using immunolocalizations with commercially available antibodies. We detected the presence of AGO proteins associated with posttranscriptional gene silencing (AGO1, 2, and 5) in the cytoplasm and the nucleus, while AGOs associated with transcriptional gene silencing (AGO4 and 9) localized exclusively in the nucleus. These results indicate that the localization of different AGOs correlates with their predicted roles at the transcriptional and posttranscriptional levels and provide an overview of their timing and potential role during meiosis.
Keywords
Meiosis; RNA silencing; Argonaute; Small RNAs; Pollen; Epigenetics
Published in
Plant Reproduction
2022, volume: 35, number: 2, pages: 153-160
Publisher: SPRINGER
SLU Authors
Martinez-Arias, Germán
- Department of Plant Biology, Swedish University of Agricultural Sciences
- Department of Plant Biology, Swedish University of Agricultural Sciences
UKÄ Subject classification
Genetics and Genomics
Publication identifier
- DOI: https://doi.org/10.1007/s00497-021-00434-z
Permanent link to this page (URI)
https://res.slu.se/id/publ/114528