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Research article - Peer-reviewed, 2021

Changes of adenosine deaminase activity in serum and saliva around parturition in sows with and without postpartum dysgalactia syndrome

Kaiser, Marianne; Dahl, Jan; Jacobsen, Stine; Jacobson, Magdalena; Andersen, Pia Haubro; Baekbo, Poul; Escribano, Damian; Ceron, Jose Joaquin; Tecles, Fernando;

Abstract

Background Postpartum dysgalactia syndrome (PDS) is associated with a significantly higher activation of the inflammatory and stress response at parturition than in the healthy sow. Therefore, reliable and possibly non-invasive biomarkers for substantial increases of inflammation are searched to support the PDS diagnosis. This report studies the possible changes of the inflammatory marker enzyme adenosine deaminase (ADA) in serum and saliva of 38 PDS positive sows (PDS+) and 38 healthy sows (PDS-). Sampling was performed every 24 h from 60 h before to 36 h after parturition. Isoenzyme 1 (ADA1) and isoenzyme 2 (ADA2), as well as total ADA (tADA), were measured and their statistical association with several serum and saliva biomarkers of inflammation and stress was investigated. Results Compared to a baseline (60 to 36h prepartum), salivary activities of ADA1, ADA2 and tADA increased significantly over time in both PDS+ and PDS- sows, reaching their peaks after parturition. In serum from PDS- sows, no changes were observed over time in either ADA1, ADA2 or tADA. In PDS+ sows, serum ADA2 activity decreased temporarily after parturition followed by a significant increase compared to baseline. ADA1, ADA2 and tADA were all significantly associated with several inflammatory biomarkers and ADA1 in serum was associated with serum cortisol. Although serum activity was higher in PDS+ than in PDS- sows, the differences were not statistically significant. Further, no difference was noted between the groups in the analyses of saliva. Conclusions Salivary ADA1 and ADA2 increased in all sows after parturition, potentially as a response to the postpartum inflammation. However, no difference in the activity of ADA1, ADA2 and tADA were found between PDS+ and PDS- sows indicating inability to diagnose PDS under the conditions described in this report.

Keywords

ADA; Inflammation; PDS; Saliva; Stress

Published in

BMC Veterinary Research

2021, volume: 17, number: 1, article number: 352
Publisher: BMC

Authors' information

Kaiser, Marianne
Aarhus University
Dahl, Jan
Danish Agriculture and Food Council
Jacobsen, Stine
University of Copenhagen
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Andersen, Pia Haubro (Haubro Andersen, Pia)
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Bækbo, Poul
SEGES
Escribano, Damian
University of Murcia
Ceron, Jose Joaquin
University of Murcia
Tecles, Fernando
University of Murcia

UKÄ Subject classification

Animal and Dairy Science

Publication Identifiers

DOI: https://doi.org/10.1186/s12917-021-03067-6

URI (permanent link to this page)

https://res.slu.se/id/publ/114538