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Research article - Peer-reviewed, 2021

Bypassing reproductive barriers in hybrid seeds using chemically induced epimutagenesis

Huc, Jonathan; Dziasek, Katarzyna; Pachamuthu, Kannan; Woh, Tristan; Köhler, Claudia; Borges, Filipe;

Abstract

Genome-wide loss of DNA methylation induced by 5-Azacytidine allows interploidy and interspecific hybridization barriers to be bypassed in Arabidopsis and Capsella.The triploid block, which prevents interploidy hybridizations in flowering plants, is characterized by a failure in endosperm development, arrest in embryogenesis, and seed collapse. Many genetic components of triploid seed lethality have been successfully identified in the model plant Arabidopsis thaliana, most notably the paternally expressed genes (PEGs), which are upregulated in tetraploid endosperm with paternal excess. Previous studies have shown that the paternal epigenome is a key determinant of the triploid block response, as the loss of DNA methylation in diploid pollen suppresses the triploid block almost completely. Here, we demonstrate that triploid seed collapse is bypassed in Arabidopsis plants treated with the DNA methyltransferase inhibitor 5-Azacytidine during seed germination and early growth. We identified strong suppressor lines showing stable transgenerational inheritance of hypomethylation in the CG context, as well as normalized expression of PEGs in triploid seeds. Importantly, differentially methylated loci segregate in the progeny of "epimutagenized" plants, which may allow epialleles involved in the triploid block response to be identified in future studies. Finally, we demonstrate that chemically induced epimutagenesis facilitates hybridization between different Capsella species, thus potentially emerging as a strategy for producing triploids and interspecific hybrids with high agronomic interest.

Published in

Plant Cell

2021, volume: 34, number: 3, pages: 989-1001

Authors' information

Huc, Jonathan
National Research Institute for Agriculture, Food and Environment (INRAE)
Swedish University of Agricultural Sciences, Department of Plant Biology
Pachamuthu, Kannan
National Research Institute for Agriculture, Food and Environment (INRAE)
Woh, Tristan
National Research Institute for Agriculture, Food and Environment (INRAE)
Swedish University of Agricultural Sciences, Department of Plant Biology
Max Planck Institute for Molecular Plant Physiology
Borges, Filipe
National Research Institute for Agriculture, Food and Environment (INRAE)

UKÄ Subject classification

Plant Biotechnology

Publication Identifiers

DOI: https://doi.org/10.1093/plcell/koab284

URI (permanent link to this page)

https://res.slu.se/id/publ/114585