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Research article - Peer-reviewed, 2021

In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19 alpha Gene Expression in Zebrafish Danio rerio

Zakariaee, Hamideh; Sudagar, Mohammad; Hosseini, Seyede Sedighe; Paknejad, Hamed; Baruah, Kartik

Abstract

In this study, we tested the compatibility of two extracts from the plant Jerusalem artichokes and button mushrooms with two different Lactobacillus probiotics (Lactobacillus acidophilus; La and Lactobacillus delbrueckii subsp. Bulgaricus; Lb) to develop a synbiotic formulation to improve the growth, survival, and reproductive performances of farmed fishes. Initially, we employed in vitro approach to monitor the growth of the probiotic lactobacilli in the presence of the different doses of the plant-based prebiotics, with the aim of selecting interesting combination(s) for further verification under in vivo conditions using zebrafish as a model. Results from the in vitro screening assay in the broth showed that both the probiotic species showed a preference for 50% mushroom extract as a source of prebiotic. A synbiotic formulation, developed with the selected combination of L. acidophilus, L. bulgaricus, and 50% mushroom extract, showed a positive influence on the growth and reproductive performances of the zebrafish. Our findings also imply that the improvement in the reproductive indices was associated with the upregulation of a cyp19a gene. Overall results suggest that a combination of L. acidophilus, L. bulgaricus, and mushroom extract can be considered as a potential synbiotic for the successful production of aquaculture species.

Keywords

prebiotics; probiotics; zebrafish; mushroom; artichoke; reproduction; cyp19a gene

Published in

Frontiers in Microbiology
2021, volume: 12, article number: 758758
Publisher: FRONTIERS MEDIA SA

Authors' information

Zakariaee, Hamideh
Gorgan Univ Agr Sci and Nat Resources
Sudagar, Mohammad
Gorgan Univ Agr Sci and Nat Resources
Hosseini, Seyede Sedighe
Golestan Univ Med Sci
Paknejad, Hamed
Gorgan Univ Agr Sci and Nat Resources
Swedish University of Agricultural Sciences, Department of Animal Nutrition and Management

UKÄ Subject classification

Medical Bioscience

Publication Identifiers

DOI: https://doi.org/10.3389/fmicb.2021.758758

URI (permanent link to this page)

https://res.slu.se/id/publ/114614