Research article - Peer-reviewed, 2021
Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling
Abreha, Kibrom B.; Alexandersson, Erik; Resjo, Svante; Lankinen, Asa; Sueldo, Daniela; Kaschani, Farnusch; Kaiser, Markus; van der Hoorn, Renier A. L.; Levander, Fredrik; Andreasson, ErikAbstract
Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural conditions. In this study, we investigated the composition of apoplastic proteomes of potato cultivar Bintje grown under field conditions, i.e., two field sites in June-August across two years and fungicide treated and untreated, using quantitative proteomics, as well as its activity using activity-based protein profiling (ABPP). Samples were clustered and some proteins showed significant intensity and activity differences, based on their field site and sampling time (June-August), indicating differential regulation of certain proteins in response to environmental or developmental factors. Peroxidases, class II chitinases, pectinesterases, and osmotins were among the proteins more abundant later in the growing season (July-August) as compared to early in the season (June). We did not detect significant differences between fungicide Shirlan treated and untreated field samples in two growing seasons. Using ABPP, we showed differential activity of serine hydrolases and beta-glycosidases under greenhouse and field conditions and across a growing season. Furthermore, the activity of serine hydrolases and beta-glycosidases, including proteins related to biotic stress tolerance, decreased as the season progressed. The generated proteomics data would facilitate further studies aiming at understanding mechanisms of molecular plant physiology in agricultural fields and help applying effective strategies to mitigate biotic and abiotic stresses.Keywords
ABPP; apoplast; proteomics; serine hydrolases; beta-glycosidases; potato; field-omicsPublished in
International Journal of Molecular Sciences2021, volume: 22, number: 21, article number: 12033
Publisher: MDPI
Authors' information
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Sueldo, Daniela
University of Oxford
Kaschani, Farnusch
University of Duisburg Essen
Kaiser, Markus
University of Duisburg Essen
van der Hoorn, Renier A. L.
University of Oxford
Levander, Fredrik
Lund University
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Associated SLU-program
SLU Network Plant Protection
UKÄ Subject classification
Biochemistry and Molecular Biology
Botany
Publication Identifiers
DOI: https://doi.org/10.3390/ijms222112033
URI (permanent link to this page)
https://res.slu.se/id/publ/114711