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Research article - Peer-reviewed, 2021

Leaf Apoplast of Field-Grown Potato Analyzed by Quantitative Proteomics and Activity-Based Protein Profiling

Abreha, Kibrom B.; Alexandersson, Erik; Resjo, Svante; Lankinen, Asa; Sueldo, Daniela; Kaschani, Farnusch; Kaiser, Markus; van der Hoorn, Renier A. L.; Levander, Fredrik; Andreasson, Erik

Abstract

Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural conditions. In this study, we investigated the composition of apoplastic proteomes of potato cultivar Bintje grown under field conditions, i.e., two field sites in June-August across two years and fungicide treated and untreated, using quantitative proteomics, as well as its activity using activity-based protein profiling (ABPP). Samples were clustered and some proteins showed significant intensity and activity differences, based on their field site and sampling time (June-August), indicating differential regulation of certain proteins in response to environmental or developmental factors. Peroxidases, class II chitinases, pectinesterases, and osmotins were among the proteins more abundant later in the growing season (July-August) as compared to early in the season (June). We did not detect significant differences between fungicide Shirlan treated and untreated field samples in two growing seasons. Using ABPP, we showed differential activity of serine hydrolases and beta-glycosidases under greenhouse and field conditions and across a growing season. Furthermore, the activity of serine hydrolases and beta-glycosidases, including proteins related to biotic stress tolerance, decreased as the season progressed. The generated proteomics data would facilitate further studies aiming at understanding mechanisms of molecular plant physiology in agricultural fields and help applying effective strategies to mitigate biotic and abiotic stresses.

Keywords

ABPP; apoplast; proteomics; serine hydrolases; beta-glycosidases; potato; field-omics

Published in

International Journal of Molecular Sciences
2021, volume: 22, number: 21, article number: 12033
Publisher: MDPI

Authors' information

Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Sueldo, Daniela
University of Oxford
Kaschani, Farnusch
University of Duisburg Essen
Kaiser, Markus
University of Duisburg Essen
van der Hoorn, Renier A. L.
University of Oxford
Levander, Fredrik
Lund University
Swedish University of Agricultural Sciences, Department of Plant Protection Biology

UKÄ Subject classification

Biochemistry and Molecular Biology
Botany

Publication Identifiers

DOI: https://doi.org/10.3390/ijms222112033

URI (permanent link to this page)

https://res.slu.se/id/publ/114711