Spent mushroom substrates for ethanol production – Effect of chemical and structural factors on enzymatic saccharification and ethanolic fermentation of Lentinula edodes-pretreated hardwood
Chen, Feng; Xiong, Shaojun; Gandla, Madhavi Latha; Stagge, Stefan; Martín, CarlosAbstract
Spent mushroom substrates (SMS) from cultivation of shiitake (Lentinula edodes) on three hardwood species were investigated regarding their potential for cellulose saccharification and for ethanolic fermentation of the produced hydrolysates. High glucan digestibility was achieved during enzymatic saccharification of the SMSs, which was related to the low mass fractions of lignin and xylan, and it was neither affected by the relative content of lignin guaiacyl units nor the substrate crystallinity. The high nitrogen content in SMS hydrolysates, which was a consequence of the fungal pretreatment, was positive for the fermentation, and it ensured ethanol yields corresponding to 84–87% of the theoretical value in fermentations without nutrient supplementation. Phenolic compounds and acetic acid were detected in the SMS hydrolysates, but due to their low concentrations, the inhibitory effect was limited. The solid leftovers resulting from SMS hydrolysis and the fermentation residues were quantified and characterized for further valorisation.
Keywords
Cellulosic ethanol; Biological pretreatment; Nitrogen; Microbial inhibitors; Resource efficiencyPublished in
Bioresource Technology2022, volume: 347, article number: 126381
Authors' information
UKÄ Subject classification
Biocatalysis and Enzyme Technology
Publication Identifiers
DOI: https://doi.org/10.1016/j.biortech.2021.126381
URI (permanent link to this page)
https://res.slu.se/id/publ/114728