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Research article - Peer-reviewed, 2021

Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda

Balinandi, Stephen; von Bromssen, Claudia; Tumusiime, Alex; Kyondo, Jackson; Kwon, Hyesoo; Monteil, Vanessa M.; Mirazimi, Ali; Lutwama, Julius; Mugisha, Lawrence; Malmberg, Maja;

Abstract

Background: Crimean-Congo Hemorrhagic Fever (CCHF) is a severe tick-borne viral hemorrhagic disease caused by Crimean-Congo Hemorrhagic Fever Virus (CCHFV) that poses serious public health challenges in many parts of Africa, Europe and Asia.Methods: We examined 500 cattle sera samples from five districts for CCHFV antibodies using in-house and commercially available (IDVet) ELISA, Immunofluorescent assay (IFA) and Real-time polymerase chain reaction (RT-PCR).Results: 500 cattle (73.8 % females) were analyzed; CCHFV seropositivity was 12.6 % (n = 63) and 75.0 % (n = 375) with the in-house and IDVet ELISAs, respectively. Seropositivity was associated with geographical location, increasing age, being female, and having a higher tick burden. Twenty four out of the 37 (64.8 %) were seropositive for CCHFV using IFA and all were negative for virus on RT-PCR. The IFA results were more com-parable to IDVet (kappa(coefficient) = 0.88, p = <0.01) than to in-house (kappa(coefficient) = 0.32, p = 0.02).Conclusions: Our study confirmed the presence and high prevalence of anti-CCHF antibodies in cattle based on three methods from all the five study districts, confirming presence and exposure of CCHFV. Given the zoonotic potential for CCHFV, we recommend a multidisciplinary public health surveillance and epidemiology of CCHFV in both animals and humans throughout the country.

Keywords

Crimean-Congo Hemorrhagic Fever Virus; Seroprevalence; Cattle; Uganda; Immunofluorescent assay

Published in

Journal of Virological Methods

2021, volume: 290, article number: 114075
Publisher: ELSEVIER

Authors' information

Balinandi, Stephen
Makerere University
von Brömssen, Claudia (Von Brömssen, Claudia)
Swedish University of Agricultural Sciences, Department of Energy and Technology
Tumusiime, Alex
Uganda Virus Research Institute
Kyondo, Jackson
Uganda Virus Research Institute
Kwon, Hyesoo
National Veterinary Institute SVA
Monteil, Vanessa M.
Karolinska University Hospital
Mirazimi, Ali
Public Health Agency of Sweden
Lutwama, Julius
Uganda Virus Research Institute
Mugisha, Lawrence
Makerere University
Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics

UKÄ Subject classification

Clinical Science

Publication Identifiers

DOI: https://doi.org/10.1016/j.jviromet.2021.114075

URI (permanent link to this page)

https://res.slu.se/id/publ/114786