Skip to main content
Research article - Peer-reviewed, 2021

Spray-Induced Gene Silencing as a Potential Tool to Control Potato Late Blight Disease

Kalyandurg, Pruthvi B.; Sundararajan, Poorva; Dubey, Mukesh; Ghadamgahi, Farideh; Zahid, Muhammad Awais; Whisson, Stephen C.; Vetukuri, Ramesh R.;

Abstract

Phytophthora infestans causes late blight disease on potato and tomato and is currently controlled by resistant cultivars or intensive fungicide spraying. Here, we investigated an alternative means for late blight control by spraying potato leaves with double-stranded RNAs (dsRNA) that target the P. infestans genes essential for infection. First, we showed that the sporangia of P. infestans expressing green fluorescent protein (GFP) can take up in vitro synthesized dsRNAs homologous to GFP directly from their surroundings, including leaves, which led to the reduced relative expression of GFP. We further demonstrate the potential of spray-induced gene silencing (SIGS) in controlling potato late blight disease by targeting developmentally important genes in P. infestans such as guanine-nucleotide binding protein beta-subunit (PiGPB1), haustorial membrane protein (PiHmp1), cutinase (PiCut3), and endo-1,3(4)-beta-glucanase (PiEndo3). Our results demonstrate that SIGS can potentially be used to mitigate potato late blight; however, the degree of disease control is dependent on the selection of the target genes.

Keywords

climate change; disease control; host parasite interactions; oomycetes; pest management

Published in

Phytopathology

2021, volume: 111, number: 12, pages: 2168-2175

Authors' information

Swedish University of Agricultural Sciences, Department of Plant Biology
Swedish University of Agricultural Sciences, Department of Plant Breeding
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Ghadamgahi, Farideh
Swedish University of Agricultural Sciences, Department of Plant Breeding
Ghadamgahi, Farideh
Ferdowsi University of Mashhad
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Whisson, Stephen C.
The James Hutton Institute
Vetukuri, Ramesh (Ramesh, Vetukuri)
Swedish University of Agricultural Sciences, Department of Plant Breeding

UKÄ Subject classification

Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.1094/PHYTO-02-21-0054-SC

URI (permanent link to this page)

https://res.slu.se/id/publ/114915