Antibiotic resistance patterns in cervical microbes of gilts and sows
Kellerman, Cecilia; Malaluang, Pongpreecha; Hansson, Ingrid; Eliasson Selling, Lena; Morrell, Jane M.Abstract
Extenders for boar semen contain antibiotics, which may induce antimicrobial resistance (AMR) in inseminated females. The objective was to investigate AMR of bacteria isolated from the cervix of sows and gilts in standing heat, representing females previously exposed to antibiotics in the semen extender and non-exposed females, respectively. Cervical swabs were taken from 30 multiparous sows and 30 gilts prior to their first insemination. After culturing on agar plates, bacterial isolates were identified by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry and antimicrobial minimum inhibitory concentrations (MIC) were determined. Differences in antibiotic resistance between sows and gilts were analyzed by Chi-squared or Fisher’s exact test. Bacteria isolated were mostly Staphylococcus spp., Streptococcus spp. and Corynebacterium spp. Higher MICs were observed for isolates from sows than from gilts. Most (>80%) Corynebacterium spp. were resistant to clindamycin; small numbers (<20%) were resistant to gentamicin, penicillin, vancomycin, ciprofloxacin and rifampicin, with no differences between gilts and sows. Corynebacterium from gilts were more often resistant to tetracycline than those from sows (25% vs. 4.17%; p = 0.04). In conclusion, bacteria from the porcine cervix showed low resistance to most antibiotics except for clindamycin, but antibacterial resistance may increase with increasing parity.
Keywords
antimicrobial resistance; vaginal flora of pigs; cervical swabs; antibiotics in semen extenders; antibiotic minimum inhibitory concentrationPublished in
Animals2022, volume: 22, number: 1, article number: 117
Authors' information
Sustainable Development Goals
SDG3 Good health and wellbeing
UKÄ Subject classification
Clinical Science
Publication Identifiers
DOI: https://doi.org/10.3390/ani12010117
URI (permanent link to this page)
https://res.slu.se/id/publ/115144