Research article - Peer-reviewed, 2021
Treatment of Respiratory Distress Syndrome with Single Recombinant Polypeptides that Combine Features of SP-B and SP-C
Basabe-Burgos, Oihana; Landreh, Michael; Rising, Anna; Curstedt, Tore; Johansson, JanAbstract
Treatment of respiratory distress syndrome (RDS) with surfactant replacement therapy in prematurely born infants was introduced more than 30 years ago; however, the surfactant preparations currently in clinical use are extracts from animal lungs. A synthetic surfactant that matches the currently used nature-derived surfactant preparations and can be produced in a cost-efficient manner would enable worldwide treatment of neonatal RDS and could also be tested against lung diseases in adults. The major challenge in developing fully functional synthetic surfactant preparations is to recapitulate the properties of the hydrophobic lung surfactant proteins B (SP-B) and SP-C. Here, we have designed single polypeptides that combine properties of SP-B and SP-C and produced them recombinantly using a novel solubility tag based on spider silk production. These Combo peptides mixed with phospholipids are as efficient as nature-derived surfactant preparations against neonatal RDS in premature rabbit fetuses.Published in
ACS Chemical Biology2021, volume: 16, number: 12, pages: 2864-2873
Publisher: AMER CHEMICAL SOC
Authors' information
Basabe-Burgos, Oihana
Karolinska Institutet
Landreh, Michael
Karolinska Institutet
Karolinska Institute
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
Curstedt, Tore
Karolinska University Hospital
Johansson, Jan
Karolinska Institutet
UKÄ Subject classification
Respiratory Medicine and Allergy
Publication Identifiers
DOI: https://doi.org/10.1021/acschembio.1c00816
URI (permanent link to this page)
https://res.slu.se/id/publ/115155