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Research article - Peer-reviewed, 2022

Norway spruce deploys tissue-specific responses during acclimation to cold

Vergara, Alexander; Haas, Julia C.; Aro, Tuuli; Stachula, Paulina; Street, Nathaniel R.; Hurry, Vaughan

Abstract

Climate change in the conifer-dominated boreal forest is expected to lead to warmer but more dynamic winter air temperatures, reducing the depth and duration of snow cover and lowering winter soil temperatures. To gain insight into the mechanisms that have enabled conifers to dominate extreme cold environments, we performed genome-wide RNA-Seq analysis from needles and roots of non-dormant two-year Norway spruce (Picea abies (L.) H. Karst), and contrasted these response to herbaceous model Arabidopsis We show that the main transcriptional response of Norway spruce needles exposed to cold was delayed relative to Arabidopsis, and this delay was associated with slower development of freezing tolerance. Despite this difference in timing, Norway spruce principally utilizes early response transcription factors (TFs) belonging to the same gene families as Arabidopsis, indicating broad evolutionary conservation of cold response networks. In keeping with their different metabolic and developmental states, needles and root of Norway spruce showed contrasting results. Regulatory network analysis identified both conserved TFs with known roles in cold acclimation (e.g. homologs of ICE1, AKS3, and of the NAC and AP2/ERF superfamilies), but also a root-specific bHLH101 homolog, providing functional insights into cold stress response strategies in Norway spruce.

Keywords

cold; Norway spruce; transcriptome

Published in

Plant, Cell and Environment
2022, volume: 45, number: 2, pages: 427-445
Publisher: WILEY

Authors' information

Vergara, Alexander
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Haas, Julia C.
Umea University
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Stachula, Paulina
Umea University
Street, Nathaniel R.
Umea University
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology

UKÄ Subject classification

Forest Science
Botany

Publication Identifiers

DOI: https://doi.org/10.1111/pce.14241

URI (permanent link to this page)

https://res.slu.se/id/publ/115161