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Research article1997Peer reviewed

A method to quantify the allelopathic compound batatasin-III in extracts from Empetrum hermaphroditum using gas chromatography: Applied on extracts from leaves of different ages

Wallstedt, A; Nilsson, MC; Odham, G; Zackrisson, O

Abstract

A gas chromatographic method was developed to quantify the allelopathic compound, batatasin-III, in organic and water extracts of leaves from the boreal dwarf shrub Empetrum hermaphroditum. The method provides a rapid and inexpensive quantification of small masses of batatasin-III in complex matrices. The compound was effectively separated from interfering compounds using solid-phase extraction techniques and chromatographed as a TMS-derivate on a nonpolar column. Batatasin-III was quantified in leaves and found to vary with the age of the shoot. The concentration of batatasin-III was highest in first- and second-year shoots, while third-year shoots contained about half that of younger shoots. Senescent leaves on older shoots still contained a considerable amount of batatasin-III. The method described provides an important tool for the demonstration of allelopathy.

Keywords

allelopathy; batatasin-III; quantification; gas chromatographic method; SPE techniques; Empetrum hermaphroditum

Published in

Journal of Chemical Ecology
1997, Volume: 23, number: 10, pages: 2345-2355
Publisher: SPRINGER

      SLU Authors

      • Zackrisson, Olle

        • Department of Forest Vegetation Ecology, Swedish University of Agricultural Sciences

      UKÄ Subject classification

      Ecology

      Publication identifier

      DOI: https://doi.org/10.1023/B:JOEC.0000006678.37973.be

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/115264