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Research article1992Peer reviewed

Isolation and Characterization of a Germination Inhibitor from Leaves of Empetrum hermaphroditum Hagerup

Oden, Per Christer; Brandtberg, Per-Olov; Andersson, Rolf; Gref, Rolf; Zackrisson, Olle; Nilsson, Marie-Charlotte

Abstract

An extract of Empetrum hermaphroditum Hagerup leaves was examined for germination inhibitors using an assay based on germination of Populus tremula L. seeds. The substances were extracted in water and purified by solvent partitioning. The acidic ethyl acetate fraction, containing the major part of the inhibitory activity was purified by reversed-phase high-performance liquid chromatography. Two zones of inhibitory activity were detected, combined and further purified by normal-phase HPLC. One major zone of inhibitory activity was thereafter detected and the substance in this fraction was crystallized and identified by nuclear magnetic resonance and gas chromatography-mass spectrometry as 5-methoxy-3,3'-dihydroxy-dihydrostilbene or batatasin III. Ca. 100 mg of the substance was then, by sequential HPLC, isolated in crystalline form from E. hermaphroditum leaves. The inhibitory activity of the pure substance was compared with a crude water extract of E. hermaphroditum leaves. It was concluded that the pure substance exhibited ca. 28 % of the inhibitory activity of the crude extract. The difference in inhibitory activity might be explained by the presence of a glucosidic conjugate of 5-methoxy-3,3'-dihydroxy-dihydrostilbene in the crude extract.

Keywords

Allelopathy; batatasin-III; bioassay; gas chromatography-mass spectrometry; 5-methoxy-3,3 '-dihydroxy-dihydrostilbene; nuclear magnetic resonance; Populus

Published in

Scandinavian Journal of Forest Research
1992, Volume: 7, number: 1-4, pages: 497-502
Publisher: TAYLOR & FRANCIS AS