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Research article2021Peer reviewedOpen access

Short-term effect of ovariohysterectomy on urine serotonin, cortisol, testosterone and progesterone in bitches

Hydbring-Sandberg, Eva; Larsson, Elin; Madej, Andrzej; Hoglund, Odd Viking


This study aimed to investigate the short-term effect of ovariohysterectomy on urine levels of serotonin and its relation to levels of cortisol, testosterone and progesterone in female dogs. Seven bitches were studied before surgical ovariohysterectomy and then once a week during 4 weeks. Spontaneously voided urine samples were collected and concentration ratios of hormone/creatinine in urine were analysed.

The bitches had significantly lower levels of cortisol, testosterone, and progesterone 1 week after ovariohysterectomy compared with before and the levels stayed low throughout the study (P ≤ 0.05). Interestingly, serotonin levels tended to increase 4 weeks after surgery (P = 0.08). A positive correlation between cortisol and progesterone was found before and after surgery. After surgery, serotonin was positively correlated with cortisol and progesterone (P ≤ 0.05).


Bitches; Canine; Cortisol; Gonadectomy; Neuter; Progesterone; Serotonin; Spay; Testosterone; Urinary hormones

Published in

BMC Research Notes
2021, Volume: 14, number: 1, article number: 265