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Research article - Peer-reviewed, 2021

New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm

Roy, Chandan; Juliana, Philomin; Kabir, Muhammad R.; Roy, Krishna K.; Gahtyari, Navin C.; Marza, Felix; He, Xinyao; Singh, Gyanendra P.; Chawade, Aakash; Joshi, Arun K.; Singh, Pawan K.

Abstract

Wheat blast (WB) disease, since its first identification in Bangladesh in 2016, is now an established serious threat to wheat production in South Asia. There is a need for sound knowledge about resistance sources and associated genomic regions to assist breeding programs. Hence, a panel of genotypes from India and Bangladesh was evaluated for wheat blast resistance and a genome-wide association study (GWAS) was performed. Disease evaluation was done during five crop seasons-at precision phenotyping platform (PPPs) for wheat blast disease at Jashore (2018-19), Quirusillas (2018-19 and 2019-20) and Okinawa (2019 and 2020). Single nucleotide polymorphisms (SNP) across the genome were obtained using DArTseq genotyping-by-sequencing platform, and in total 5713 filtered markers were used. GWAS revealed 40 significant markers associated with WB resistance, of which 33 (82.5%) were in the 2NS/2AS chromosome segment and one each on seven chromosomes (3B, 3D, 4A, 5A, 5D, 6A and 6B). The 2NS markers contributed significantly in most of the environments, explaining an average of 33.4% of the phenotypic variation. Overall, 22.4% of the germplasm carried 2NS/2AS segment. So far, 2NS translocation is the only effective WB resistance source being used in the breeding programs of South Asia. Nevertheless, the identification of non-2NS/2AS genomic regions for WB resistance provides a hope to broaden and diversify resistance for this disease in years to come.

Keywords

GWAS; SNPs; genotyping-by-sequencing; wheat blast

Published in

Plants
2021, volume: 10, article number: 2693
Publisher: MDPI

Authors' information

Roy, Chandan
Bihar Agricultural University
Juliana, Philomin
BISA CIMMYT India
Kabir, Muhammad R.
Bangladesh Wheat and Maize Research Institute
Roy, Krishna K.
Bangladesh Wheat and Maize Research Institute
Gahtyari, Navin C.
Indian Council of Agricultural Research (ICAR)
Marza, Felix
Inst Nacl Innovac Agr and Forestal INIAF
He, Xinyao
International Maize and Wheat Improvement Center (CIMMYT)
Singh, Gyanendra P.
Indian Council of Agricultural Research (ICAR)
Swedish University of Agricultural Sciences, Department of Plant Breeding
Joshi, Arun K.
International Maize and Wheat Improvement Center (CIMMYT)
Singh, Pawan K.
International Maize and Wheat Improvement Center (CIMMYT)

UKÄ Subject classification

Genetics and Breeding

Publication Identifiers

DOI: https://doi.org/10.3390/plants10122693

URI (permanent link to this page)

https://res.slu.se/id/publ/115766