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Research article2002Peer reviewed

Characterization of polymorphic microsatellite DNA markers in the ruff (Philomachus pugnax)

Thuman, KA; Widemo, F; Piertney, SB

Abstract

Nine polymorphic microsatellite markers were isolated and characterized from the lek-breeding ruff (Philomachus pugnax) using an enrichment cloning procedure. The number of alleles resolved per locus ranged from five to 56, and observed heterozygosity ranged from 0.311 to 0.857 in a sample of 319 ruffs from Gotland, Sweden. Two loci were located on the Z chromosome, rendering all females hemizygotes. These markers are effective in the assignment of parentage and determining male mating success.

Keywords

enrichment; microsatellite; paternity; Philomachus; ruff; sex-linked

Published in

Molecular Ecology Notes
2002, Volume: 2, number: 3, pages: 276-277
Publisher: WILEY

    UKÄ Subject classification

    Ecology

    Publication identifier

    DOI: https://doi.org/10.1046/j.1471-8286.2002.00221.x-i2

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/116036