Skip to main content
Research article - Peer-reviewed, 2022

Anti-attractant activity of (+)-trans-4-thujanol for Eurasian spruce bark beetle Ips typographus: Novel potency for females

Jirosova, Anna; Kalinova, Blanka; Modlinger, Roman; Jakus, Rastislav; Unelius, C. Rikard; Blazenec, Miroslav; Schlyter, Fredrik


BACKGROUND Eurasian spruce bark beetles (Ips typographus) use both attractant and anti-attractant semiochemicals to find suitable mature host trees. Trans-4-thujanol is abundant in young, unsuitable spruce trees. Electrophysiological studies have demonstrated its high activity levels, but field data are lacking. RESULTS Enantioselective GC-MS analysis showed that only (1R,4S)-(+)-trans-4-thujanol was present in Norway spruce bark volatiles. In a factorial design field-trapping experiment, trans-4-thujanol alone was not attractive to Ips typographus. Traps baited with I. typographus' aggregation pheromone and trans-4-thujanol or the known anti-attractant 1,8-cineole caught fewer beetles than those baited with the aggregation pheromone alone. Catches for trans-4-thujanol and 1,8-cineole were dose-dependent. Intermediate doses of trans-4-thujanol and 1,8-cineole had a similar effect. Surprisingly, in contrast to 1,8-cineole and other known Ips anti-attractants, which all inhibit males more strongly than females, the addition of trans-4-thujanol to the aggregation pheromone reduced the attraction of females more. CONCLUSION The Norway spruce volatile (+)-trans-4-thujanol is a novel I. typographus anti-attractant with potency comparable to the known anti-attractants 1,8-cineole and verbenone, and is more effective for females than for males. Incorporating (+)-trans-4-thujanol into anti-attractant lures could improve protection of trees from mass attack by I. typographus.


Coleoptera; Curculionidae; Scolytinae; repellent; (1R; 4S)-(+)-trans-4-thujanol; sabinene hydrate; dose-response; oxygenated terpene; integrated pest management

Published in

Pest Management Science
2022, volume: 78, number: 5, pages: 1992-1999

Authors' information

Jirosova, Anna
Czech University of Life Sciences Prague
Kalinova, Blanka
Czech University of Life Sciences Prague
Modlinger, Roman
Czech University of Life Sciences Prague
Jakus, Rastislav
Slovak Academy of Sciences
Unelius, C. Rikard
Linnaeus University
Blazenec, Miroslav
Slovak Academy of Sciences
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Czech University of Life Sciences Prague

UKÄ Subject classification

Forest Science

Publication Identifiers


URI (permanent link to this page)