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Research article - Peer-reviewed, 2021

FERONIA and microtubules independently contribute to mechanical integrity in the Arabidopsis shoot

Malivert, Alice; Erguvan, Ozer; Chevallier, Antoine; Dehem, Antoine; Friaud, Rodrigue; Liu, Mengying; Martin, Marjolaine; Peyraud, Theophile; Hamant, Olivier; Verger, Stephane

Abstract

To survive, cells must constantly resist mechanical stress. In plants, this involves the reinforcement of cell walls, notably through microtubule-dependent cellulose deposition. How wall sensing might contribute to this response is unknown. Here, we tested whether the microtubule response to stress acts downstream of known wall sensors. Using a multistep screen with 11 mutant lines, we identify FERONIA (FER) as the primary candidate for the cell's response to stress in the shoot. However, this does not imply that FER acts upstream of the microtubule response to stress. In fact, when performing mechanical perturbations, we instead show that the expected microtubule response to stress does not require FER. We reveal that the feronia phenotype can be partially rescued by reducing tensile stress levels. Conversely, in the absence of both microtubules and FER, cells appear to swell and burst. Altogether, this shows that the microtubule response to stress acts as an independent pathway to resist stress, in parallel to FER. We propose that both pathways are required to maintain the mechanical integrity of plant cells.

Published in

PLoS Biology
2021, volume: 19, number: 11, article number: e3001454
Publisher: PUBLIC LIBRARY SCIENCE

Authors' information

Malivert, Alice
Claude Bernard University Lyon 1
Erguvan, Ozer
Claude Bernard University Lyon 1
Chevallier, Antoine
Claude Bernard University Lyon 1
Dehem, Antoine
Claude Bernard University Lyon 1
Friaud, Rodrigue
Claude Bernard University Lyon 1
Liu, Mengying
Claude Bernard University Lyon 1
Martin, Marjolaine
Claude Bernard University Lyon 1
Peyraud, Theophile
Claude Bernard University Lyon 1
Hamant, Olivier
Claude Bernard University Lyon 1
Verger, Stephane
Claude Bernard University Lyon 1

UKÄ Subject classification

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Publication Identifiers

DOI: https://doi.org/10.1371/journal.pbio.3001454

URI (permanent link to this page)

https://res.slu.se/id/publ/116315