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Research article - Peer-reviewed, 2022

Whole-genome sequencing elucidates the species-wide diversity and evolution of fungicide resistance in the early blight pathogen Alternaria solani

Einspanier, Severin; Susanto, Tamara; Metz, Nicole; Wolters, Pieter J.; Vleeshouwers, Vivianne G. A. A.; Lankinen, Asa; Liljeroth, Erland; Landschoot, Sofie; Ivanovic, Zarko; Hueckelhoven, Ralph; Hausladen, Hans; Stam, Remco;

Abstract

Early blight of potato is caused by the fungal pathogen Alternaria solani and is an increasing problem worldwide. The primary strategy to control the disease is applying fungicides such as succinate dehydrogenase inhibitors (SDHI). SDHI-resistant strains, showing reduced sensitivity to treatments, appeared in Germany in 2013, shortly after the introduction of SDHIs. Two primary mutations in the SDH complex (SdhB-H278Y and SdhC-H134R) have been frequently found throughout Europe. How these resistances arose and spread, and whether they are linked to other genomic features, remains unknown. For this project, we performed whole-genome sequencing for 48 A. solani isolates from potato fields across Europe to better characterize the pathogen's genetic diversity in general and understand the development and spread of the genetic mutations that lead to SDHI resistance. The isolates can be grouped into seven genotypes. These genotypes do not show a geographical pattern but appear spread throughout Europe. We found clear evidence for recombination on the genome, and the observed admixtures might indicate a higher adaptive potential of the fungus than previously thought. Yet, we cannot link the observed recombination events to different Sdh mutations. The same Sdh mutations appear in different, non-admixed genetic backgrounds; therefore, we conclude they arose independently. Our research gives insights into the genetic diversity of A. solani on a genome level. The mixed occurrence of different genotypes, apparent admixture in the populations, and evidence for recombination indicate higher genomic complexity than anticipated. The conclusion that SDHI tolerance arose multiple times independently has important implications for future fungicide resistance management strategies. These should not solely focus on preventing the spread of isolates between locations but also on limiting population size and the selective pressure posed by fungicides in a given field to avoid the rise of new mutations in other genetic backgrounds.

Keywords

agriculture; alternaria solani; fungicide resistance; plant pathology; population genetics; empirical; potato

Published in

Evolutionary applications

2022, volume: 15, number: 10, pages: 1605-1620
Publisher: WILEY

Authors' information

Einspanier, Severin
Technical University of Munich
Susanto, Tamara
Technical University of Munich
Metz, Nicole
Technical University of Munich
Wolters, Pieter J.
Wageningen University and Research
Vleeshouwers, Vivianne G. A. A.
Wageningen University and Research
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Landschoot, Sofie
Ghent University
Ivanović, Žarko
Institute for Plant Protection and Environment (IPPE)
Hueckelhoven, Ralph
Technical University of Munich
Hausladen, Hans
Technical University of Munich
Stam, Remco
Technical University of Munich

UKÄ Subject classification

Agricultural Science
Evolutionary Biology

Publication Identifiers

DOI: https://doi.org/10.1111/eva.13350

URI (permanent link to this page)

https://res.slu.se/id/publ/116351