Research article - Peer-reviewed, 2022
Application of a novel prioritisation strategy using non-target screening for evaluation of temporal trends (1969-2017) of contaminants of emerging concern (CECs) in archived lynx muscle tissue samples
Durig, Wiebke; Alygizakis, Nikiforos A.; Wiberg, Karin; Ahrens, LutzAbstract
Most environmental monitoring studies of contaminants of emerging concern (CECs) focus on aquatic species and target specific classes of CECs. Even with wide-scope target screening methods, relevant CECs may be missed. In this study, non-target screening (NTS) was used for tentative identification of potential CECs in muscle tissue of the terrestrial top predator Eurasian lynx (Lynx lynx). Temporal trend analysis was applied as a prioritisation tool for archived samples, using univariate statistical tests (Mann-Kendall and Spearman rank). Pooled lynx muscle tissue collected from 1969 to 2017 was analysed with an eight-point time series using a previously validated screening workflow. Following peak detection, peak alignment, and blank subtraction, 12,941 features were considered for statistical analysis. Prioritisation by time-trend analysis detected 104 and 61 features with statistically significant increasing and decreasing trends, respectively. Following probable molecular formula assignment and elucidation with MetFrag, two compounds with increasing trends, and one with a decreasing trend, were tentatively identified. These results show that, despite low expected concentration levels and high matrix effects in terrestrial species, it is possible to prioritise CECs in archived lynx samples using NTS and univariate statistical approaches.Keywords
Non-target analysis; Environmental specimen bank; lynx; LC-QToF-MS; Temporal trend analysisPublished in
Science of the Total Environment2022, volume: 817, article number: 153035
Publisher: ELSEVIER
Authors' information
Dürig, Wiebke
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment
Alygizakis, Nikiforos A.
National and Kapodistrian University of Athens
Alygizakis, Nikiforos A.
Environmental Institute
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment
Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment
UKÄ Subject classification
Environmental Sciences
Publication Identifiers
DOI: https://doi.org/10.1016/j.scitotenv.2022.153035
URI (permanent link to this page)
https://res.slu.se/id/publ/116549