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Research article - Peer-reviewed, 2022

Unraveling the Genetic Basis of Key Agronomic Traits of Wrinkled Vining Pea (Pisum sativum L.) for Sustainable Production

Abebe, Admas Alemu; Kolodinska Brantestam, Agnese; Chawade, Aakash

Abstract

Estimating the allelic variation and exploring the genetic basis of quantitatively inherited complex traits are the two foremost breeding scenarios for sustainable crop production. The current study utilized 188 wrinkled vining pea genotypes comprising historical varieties and breeding lines to evaluate the existing genetic diversity and to detect molecular markers associated with traits relevant to vining pea production, such as wrinkled vining pea yield (YTM100), plant height (PH), earliness (ERL), adult plant resistance to downy mildew (DM), pod length (PDL), numbers of pods per plant (PDP), number of peas per pod (PPD), and percent of small wrinkled vining peas (PSP). Marker-trait associations (MTAs) were conducted using 6902 quality single nucleotide polymorphism (SNP) markers generated from the diversity arrays technology sequencing (DArTseq) and Genotyping-by-sequencing (GBS) sequencing methods. The best linear unbiased prediction (BLUP) values were estimated from the two-decades-long (1999-2020) unbalanced phenotypic data sets recorded from two private breeding programs, the Findus and the Birds eye, now owned by Nomad Foods. Analysis of variance revealed a highly significant variation between genotypes and genotype-by-environment interactions for the ten traits. The genetic diversity and population structure analyses estimated an intermediate level of genetic variation with two optimal sub-groups within the current panel. A total of 48 significant (P < 0.0001) MTAs were identified for eight different traits, including five for wrinkled vining pea yield on chr2LG1, chr4LG4, chr7LG7, and scaffolds (two), and six for adult plant resistance to downy mildew on chr1LG6, chr3LG5 (two), chr6LG2, and chr7LG7 (two). We reported several novel MTAs for different crucial traits with agronomic importance in wrinkled vining pea production for the first time, and these candidate markers could be easily validated and integrated into the active breeding programs for marker-assisted selection.

Keywords

wrinkled vining pea yield; downy mildew; DArTseq; GWAS; GBS

Published in

Frontiers in Plant Science
2022, volume: 13, article number: 844450

Authors' information

Swedish University of Agricultural Sciences, Department of Plant Breeding
Kolodinska Brantestam, Agnese
Nomad Foods
Swedish University of Agricultural Sciences, Department of Plant Breeding

Sustainable Development Goals

SDG2 Zero hunger
SDG12 Ensure sustainable consumption and production patterns

UKÄ Subject classification

Genetics and Breeding

Publication Identifiers

DOI: https://doi.org/10.3389/fpls.2022.844450

URI (permanent link to this page)

https://res.slu.se/id/publ/116560