Transformation systems, gene silencing and gene editing technologies in oomycetes

Abstract

Oomycetes are spore-forming eukaryotic microbes responsible for infections in animal and plant species worldwide, posing a threat to natural ecosystems, biodiversity and food security. Genomics and transcriptomics approaches, together with host interaction studies, give promising results towards better understanding of the infection mechanisms in oomycetes and their general biology. Significant development and progress in oomycetes genomic studies have been achieved over the past decades but further understanding of molecular processes, gene regulations and infection mechanisms are still needed. The use of molecular tools such as CRISPR/Cas and RNAi helped elucidate some of the molecular processes involved in host invasion and infection both in plant and animal pathogenic oomycetes. These methods provide an opportunity for accurate and detailed functional analysis involving various fields of studies such as genomics, epigenomics, proteomics, and interactomics. Functional gene characterisation is essential for filling the knowledge gaps in dynamic biological processes. However, every method has both advantages and limitations that should be considered before choosing the best method for investigating a particular research question. Here we review transformation systems, gene silencing and gene editing techniques in oomycetes, how they function, in which species and what are their main advantages and disadvantages. (C) 2021 The Author(s). Published by Elsevier Ltd on behalf of British Mycological Society.

Keywords

CRISPR/Cas; Gene editing; Gene silencing; Oomycete pathogens; Transformation techniques

Published in

Fungal Biology Reviews
2022, volume: 40, pages: 37-52

SLU Authors

• Andersen, Christian Benjamin

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

Global goals (SDG)

SDG15 Life on land

UKÄ Subject classification

Agricultural Science

Publication identifier

• DOI: https://doi.org/10.1016/j.fbr.2021.11.001

Permanent link to this page (URI)

https://res.slu.se/id/publ/116699