Tigabu, Mulualem
- Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences
Abstract
Acer pseudosieboldianum (Pax) Komarov is an ornamental plant with prominent potential and is naturally distributed in Northeast China. Here, we obtained a chromosome-scale genome assembly of A. pseudosieboldianum combining HiFi and Hi-C data, and the final assembled genome size was 690.24 Mb and consisted of 287 contigs, with a contig N50 value of 5.7 Mb and a BUSCO complete gene percentage of 98.4%. Genome evolution analysis showed that an ancient duplication occurred in A. pseudosieboldianum. Phylogenetic analyses revealed that Aceraceae family could be incorporated into Sapindaceae, consistent with the present Angiosperm Phylogeny Group system. We further construct a gene-to-metabolite correlation network and identified key genes and metabolites that might be involved in anthocyanin biosynthesis pathways during leaf color change. Additionally, we identified crucial teosinte branched1, cycloidea, and proliferating cell factors (TCP) transcription factors that might be involved in leaf morphology regulation of A. pseudosieboldianum, Acer yangbiense and Acer truncatum. Overall, this reference genome is a valuable resource for evolutionary history studies of A. pseudosieboldianum and lays a fundamental foundation for its molecular breeding.
Keywords
Acer pseudosieboldianum; PacBio SMRT; HiFi; genome assembly; Hi-C; TCPs
Published in
Frontiers in Plant Science
2022, volume: 13, article number: 850054
Publisher: FRONTIERS MEDIA SA
SLU Authors
UKÄ Subject classification
Forest Science
Bioinformatics and Computational Biology (Methods development to be 10203)
Publication identifier
- DOI: https://doi.org/10.3389/fpls.2022.850054
Permanent link to this page (URI)
https://res.slu.se/id/publ/116843