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Research article - Peer-reviewed, 2022

ZEITLUPE Promotes ABA-Induced Stomatal Closure in Arabidopsis and Populus

Jurca, Manuela; Sjoelander, Johan; Ibanez, Cristian; Matrosova, Anastasia; Johansson, Mikael; Kozarewa, Iwanka; Takata, Naoki; Bako, Laszlo; Webb, Alex A. R.; Israelsson-Nordstroem, Maria; Eriksson, Maria E.;

Abstract

Plants balance water availability with gas exchange and photosynthesis by controlling stomatal aperture. This control is regulated in part by the circadian clock, but it remains unclear how signalling pathways of daily rhythms are integrated into stress responses. The serine/threonine protein kinase OPEN STOMATA 1 (OST1) contributes to the regulation of stomatal closure via activation of S-type anion channels. OST1 also mediates gene regulation in response to ABA/drought stress. We show that ZEITLUPE (ZTL), a blue light photoreceptor and clock component, also regulates ABA-induced stomatal closure in Arabidopsis thaliana, establishing a link between clock and ABA-signalling pathways. ZTL sustains expression of OST1 and ABA-signalling genes. Stomatal closure in response to ABA is reduced in ztl mutants, which maintain wider stomatal apertures and show higher rates of gas exchange and water loss than wild-type plants. Detached rosette leaf assays revealed a stronger water loss phenotype in ztl-3, ost1-3 double mutants, indicating that ZTL and OST1 contributed synergistically to the control of stomatal aperture. Experimental studies of Populus sp., revealed that ZTL regulated the circadian clock and stomata, indicating ZTL function was similar in these trees and Arabidopsis. PSEUDO-RESPONSE REGULATOR 5 (PRR5), a known target of ZTL, affects ABA-induced responses, including stomatal regulation. Like ZTL, PRR5 interacted physically with OST1 and contributed to the integration of ABA responses with circadian clock signalling. This suggests a novel mechanism whereby the PRR proteins-which are expressed from dawn to dusk-interact with OST1 to mediate ABA-dependent plant responses to reduce water loss in time of stress.

Keywords

abiotic stress; abscisic acid; circadian clock; stomatal closure; ZEITLUPE; OPEN STOMATA 1; PSEUDO-RESPONSE REGULATORs

Published in

Frontiers in Plant Science

2022, volume: 13, article number: 829121
Publisher: FRONTIERS MEDIA SA

Authors' information

Jurca, Manuela
Umea University
Sjoelander, Johan
Umea University
Ibanez, Cristian
Umea University
Ibanez, Cristian
Universidad de La Serena
Matrosova, Anastasia
Swedish University of Agricultural Sciences, Department of Forest Genetics and Plant Physiology
Johansson, Mikael
Umea University
Johansson, Mikael
University of Bielefeld
Kozarewa, Iwanka
Umea University
Takata, Naoki
Umea University
Takata, Naoki
Forestry and Forest Products Research Institute - Japan
Bako, Laszlo
Umea University
Webb, Alex A. R.
University of Cambridge
Eriksson, Maria E.
Umea University
Eriksson, Maria E.
University of Cambridge

UKÄ Subject classification

Botany

Publication Identifiers

DOI: https://doi.org/10.3389/fpls.2022.829121

URI (permanent link to this page)

https://res.slu.se/id/publ/116844